Abstract | BACKGROUND: METHODS: RESULTS: Immunostaining for H(1) and H(2) receptors was slightly positive in normal and over-expressed in vernal tissues. H(3) receptors were rarely present in normal and inflamed conjunctiva. In striking contrast to control tissues, H(4) receptors were highly expressed in all inflamed tissues, particularly by stromal inflammatory cells. Semi-quantitative reverse-transcriptase polymerase chain reaction demonstrated an over-expression of H(1), H(2), and H(4) receptors in vernal vs control tissues. Notably, H(4) receptors were five times more expressed in vernal vs control tissues. In cell cultures, H(2) receptor expression was stimulated eight times the normal levels by interleukin-4 and three times by histamine, but the H(4) receptor was only slightly affected by stimulation with these mediators. CONCLUSIONS: Increased expression of H1, and particularly of H(2) and H(4) receptors in vernal keratoconjunctival tissues indicate their important role in the pathogenesis of this disease. H(4) receptors may be a target in the treatment of allergic inflammation.
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Authors | A Leonardi, A Di Stefano, C Vicari, L Motterle, P Brun |
Journal | Allergy
(Allergy)
Vol. 66
Issue 10
Pg. 1360-6
(Oct 2011)
ISSN: 1398-9995 [Electronic] Denmark |
PMID | 21545429
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | © 2011 John Wiley & Sons A/S. |
Chemical References |
- HRH4 protein, human
- RNA, Messenger
- Receptors, G-Protein-Coupled
- Receptors, Histamine
- Receptors, Histamine H4
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Topics |
- Adolescent
- Child
- Conjunctiva
(metabolism)
- Conjunctivitis, Allergic
(genetics, metabolism)
- Female
- Gene Expression Regulation
- Humans
- Male
- RNA, Messenger
- Receptors, G-Protein-Coupled
(metabolism)
- Receptors, Histamine
(metabolism)
- Receptors, Histamine H4
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