In vitro susceptibility of Pythium insidiosum to macrolides and tetracycline antibiotics.

Abstract

We describe the in vitro activity of macrolides and tetracycline antibiotics against Pythium insidiosum. The MICs were determined according to CLSI procedures (visual MIC) and by a colorimetric method [3-(4,5-dimethyl-2-thiazyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT)]. The lowest geometric mean (GM) MIC (MICs in μg/ml) (0.39 and 0.7 by visual reading and colorimetric method, respectively) and MIC ranges (0.125 to 2.0) were obtained for minocycline, while the highest MICs were shown for erythromycin (GM of 7.58 and 12.25 by visual reading and colorimetric method, respectively, and MIC ranged from 2 to 32). This significant in vitro activity makes these classes of antibiotics good candidates for experimental treatment of pythiosis.

• Authors: Erico Silva Loreto, Débora Alves Nunes Mario, Laura Bedin Denardi, Sydney Hartz Alves, Janio Morais Santurio
• Journal: Antimicrobial agents and chemotherapy (Antimicrob Agents Chemother) Vol. 55 Issue 7 Pg. 3588-90 (Jul 2011) ISSN: 1098-6596 [Electronic] United States
• PMID: 21537028 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Anti-Bacterial Agents
• Macrolides
• Erythromycin
• Tetracycline
• Minocycline

Topics

• Anti-Bacterial Agents (pharmacology)
• Erythromycin (pharmacology)
• Macrolides (pharmacology)
• Microbial Sensitivity Tests
• Minocycline (pharmacology)
• Pythium (drug effects)
• Tetracycline (pharmacology)