Abstract | OBJECTIVE: ANIMALS: 5 healthy adult horses. PROCEDURES: Horses were anesthetized, and two 30-cm segments of jejunum were surgically exteriorized. Blood flow was maintained at baseline (untreated) values in 1 (control) segment and was decreased to 20% of baseline (low-flow ischemia) for 75 minutes, followed by 75 minutes of reperfusion, in the other (experimental) segment. Biopsy samples were collected from experimental segments at baseline (T0), after 75 minutes of ischemia (T1), and after 75 minutes of reperfusion (T2); samples were collected from control segments at T0 and T2. Horses were euthanized 24 hours after induction of ischemia (T3), and additional samples were collected. Samples were evaluated histologically. Total RNA was extracted; expression of COX genes and stability of 8 housekeeping genes were determined via quantitative real-time PCR assays. RESULTS: CONCLUSIONS AND CLINICAL RELEVANCE: Low-flow ischemia resulted in upregulation of COX-2 gene expression in the jejunum of horses. Housekeeping genes traditionally used as internal standards may not be stable in this tissue during arterial low-flow ischemia and reperfusion.
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Authors | Hugo Hilton, Jorge E Nieto, Peter F Moore, Faye A Harmon, Diane K Naydan, Jack R Snyder |
Journal | American journal of veterinary research
(Am J Vet Res)
Vol. 72
Issue 5
Pg. 681-6
(May 2011)
ISSN: 1943-5681 [Electronic] United States |
PMID | 21529221
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Cyclooxygenase 1
- Cyclooxygenase 2
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Topics |
- Animals
- Cyclooxygenase 1
(genetics)
- Cyclooxygenase 2
(genetics)
- Female
- Gene Expression Regulation, Enzymologic
- Horse Diseases
(metabolism, pathology)
- Horses
- Ischemia
(metabolism, veterinary)
- Jejunal Diseases
(metabolism, pathology, veterinary)
- Jejunum
(blood supply, metabolism, pathology)
- Male
- Reperfusion Injury
(metabolism, veterinary)
- Up-Regulation
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