Abstract |
In the total fraction of low and very low density lipoproteins (LDL+VLDL) isolated from serum by precipitation in the presence of heparin-Mn the copper-induced lipid peroxidation was accompanied by accumulation of LPO products, a decrease ANS fluorescence intensity (F(ANS)) and an increase in probe-- cation DSP-6, a fluorescence intensity decrease of intrinsic in the ultraviolet area (F(uv)) and an increase in the visible area (F(vis)). The degree of lipoprotein modification was estimated by calculating the F(vis)/F(uv) and F(DSP)-6/F(ANS) ratio. Strong positive correlation was found between these ratios and concentration of thiobarbituric acid-reactive substances ( TBARS) of LDL+VLDL samples isolated from sera of 49 donors and incubated at 37 degrees C in the presence of 50 M CuSO4 during 0, 3 and 24 hr (F(vis)/F(uv) (r = 0.75; p < 0.001) and F(DSP-6)/F(ANS) (r = 0.73; p < 0.001)). Very strong positive correlation was also found between both fluorescent parameters F(vis)/F(uv) and F(DSP-6)/F(ANS) (r = 0.95, p < 0.001). Changes in the values of F(vis)/ F(uv), F(DSP-6)/F(ANS), concentration of TBARS in 75 patients with documented coronary heart disease (CHD) and 49 apparently healthy donors were studied. No significant differences of these parameters in LDL+VLDL of patients with CHD and donors were found.
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Authors | R K Aĭdyraliev, O A Igemberdieva, M Kh Dadabaev, T M Murataliev, K A Aĭtbaev, A A Aldashev |
Journal | Biomeditsinskaia khimiia
(Biomed Khim)
2011 Jan-Feb
Vol. 57
Issue 1
Pg. 127-32
ISSN: 2310-6972 [Print] Russia (Federation) |
PMID | 21516784
(Publication Type: English Abstract, Journal Article)
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Chemical References |
- Fibrinolytic Agents
- Lipoproteins, LDL
- Lipoproteins, VLDL
- Thiobarbituric Acid Reactive Substances
- Manganese
- Copper
- Heparin
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Topics |
- Copper
(pharmacology)
- Coronary Disease
(blood)
- Female
- Fibrinolytic Agents
(pharmacology)
- Heparin
(pharmacology)
- Humans
- Lipid Peroxidation
(drug effects)
- Lipoproteins, LDL
(blood, chemistry)
- Lipoproteins, VLDL
(blood, chemistry)
- Male
- Manganese
(pharmacology)
- Spectrometry, Fluorescence
- Thiobarbituric Acid Reactive Substances
(analysis, chemistry)
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