Abstract | BACKGROUND: The most important factor limiting the success of an antiretroviral therapy is toxicity. The HLA-B*5701 allele is predictive of hypersensitivity reaction to Abacavir, and this gene is in a perfect linkage disequilibrium with the rs2395029 SNP present in the HCP5 gene. METHODS: Genomic DNA was extracted from blood obtained from 201 unrelated healthy Argentinean volunteers. The DNA was subjected to an allele-specific PCR method. Sequencing was performed to validate the test results. RESULTS: We were successful to amplify specific fragment of interest from the DNA samples. The method is easy, specific and reproducible. CONCLUSIONS: The application of this methodology is a rapid and simple method to detect the HCP5 polymorphism (rs2395029) previous to administration of Abacavir in patients with HIV infection.
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Authors | Cristian A Galván, Osvaldo C Elbarcha, Eduardo J Fernández, Dante M Beltramo, Néstor Walter Soria |
Journal | Clinica chimica acta; international journal of clinical chemistry
(Clin Chim Acta)
Vol. 412
Issue 15-16
Pg. 1382-4
(Jul 15 2011)
ISSN: 1873-3492 [Electronic] Netherlands |
PMID | 21514285
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2011 Elsevier B.V. All rights reserved. |
Chemical References |
- Anti-HIV Agents
- Dideoxynucleosides
- HCP5 long noncoding RNA, human
- RNA, Long Noncoding
- RNA, Untranslated
- abacavir
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Topics |
- Adolescent
- Adult
- Aged
- Alleles
- Anti-HIV Agents
(adverse effects)
- Dideoxynucleosides
(adverse effects)
- Drug Hypersensitivity
(blood, genetics)
- Female
- Genotype
- Humans
- Major Histocompatibility Complex
(genetics)
- Male
- Middle Aged
- Polymerase Chain Reaction
- Polymorphism, Single Nucleotide
(genetics)
- Predictive Value of Tests
- RNA, Long Noncoding
- RNA, Untranslated
- Reference Values
- Reproducibility of Results
- Sensitivity and Specificity
- Young Adult
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