Chemopreventive effect of mofezolac on beef tallow diet/azoxymethane-induced colon carcinogenesis in rats.

Abstract

BACKGROUND/AIMS:

We have previously shown that long-term consumption of 10% beef tallow diet promotes colon carcinogenesis in both saline- and azoxymethane (AOM)-treated rats. Here, we investigated the effects of mofezolac, a selective COX-1 inhibitor, on beef tallow-fed rats with saline- or AOM treatment.

METHODOLOGY:

Male SD rats were intraperitoneally injected with saline or AOM and fed 10% beef tallow diet with or without 1200 ppm mofezolac. At 12 weeks, aberrant crypt foci (ACF) were examined. At 44 weeks, tumors were counted, the proliferation and expression of COX-1 and 8-catenin on normal-appearing colonic mucosa was evaluated using the BrdU incorporation assay and Western blotting respectively.

RESULTS:

Mofezolac decreased the number of ACF at 12 weeks (p < 0.05) and reduced tumor multiplicity and incidence at 44 weeks in beef tallow-fed rats with AOM treatment (p < 0.05). At 44 weeks, reduction of the BrdU-positive cells (p < 0.05) and beneficial distribution changes of these cells within the colon crypts in both groups with mofezolac supplementation were observed. The expression of COX-1 and beta-catenin also reduced in mofezolac-added groups simultaneously (p < 0.05).

CONCLUSIONS:

This study suggested that mofezolac suppressed beef tallow-promoted colon carcinogenesis in rats, which probably was, appropriate for populations with high fat intake.

• Authors: Lujie Miao, Ryosuke Shiraishi, Takehiro Fujise, Tsukasa Kuroki, Takashi Kakimoto, Yasuhisa Sakata, Tooru Takashima, Ryuichi Iwakiri, Kazuma Fujimoto, Ruihua Shi, Xueliang Li
• Journal: Hepato-gastroenterology (Hepatogastroenterology) 2011 Jan-Feb Vol. 58 Issue 105 Pg. 81-8 ISSN: 0172-6390 [Print] Greece
• PMID: 21510291 (Publication Type: Journal Article)

Chemical References

• Dietary Fats
• Fats
• Isoxazoles
• tallow
• Azoxymethane
• mofezolac

Topics

• Animals
• Azoxymethane (toxicity)
• Blotting, Western
• Cattle
• Cell Proliferation (drug effects)
• Chi-Square Distribution
• Colonic Neoplasms (etiology, prevention & control)
• Dietary Fats (toxicity)
• Fats
• Isoxazoles (administration & dosage, pharmacology)
• Male
• Rats
• Rats, Sprague-Dawley