Abstract | OBJECTIVE: TREATMENT: METHODS: TGF-β1 was determined by enzyme-linked immonosorbent assay. STAT3-DNA binding activity was measured by electrophoretic mobility shift assay. Levels of MAPKs as well as total and phospho-specific forms of Jak1/Stat3 were assessed by Western blot analysis. RESULTS:
Cerulein induced increases in TGF-β1, Stat3-DNA binding activity and the activation of MAPKs in AR42J cells. AG490 suppressed these cerulein-induced changes, similar to inhibition by DPI. Cerulein-induced activation of Jak2/Stat3 and increases in MAPKs and TGF-β1 levels were inhibited in the cells transfected with AS ODN for p22( phox) and p47( phox) compared to S ODN controls. CONCLUSION: Inhibition of NADPH oxidase may be beneficial for prevention and treatment of pancreatitis by suppressing Jak2/Stat3 and MAPKs and expression of TGF-β1 in pancreatic acinar cells.
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Authors | Kyung Don Ju, Joo Weon Lim, Kyung Hwan Kim, Hyeyoung Kim |
Journal | Inflammation research : official journal of the European Histamine Research Society ... [et al.]
(Inflamm Res)
Vol. 60
Issue 8
Pg. 791-800
(Aug 2011)
ISSN: 1420-908X [Electronic] Switzerland |
PMID | 21509626
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Enzyme Inhibitors
- Reactive Oxygen Species
- STAT3 Transcription Factor
- Transforming Growth Factor beta1
- Tyrphostins
- alpha-cyano-(3,4-dihydroxy)-N-benzylcinnamide
- Ceruletide
- NADPH Oxidases
- Janus Kinase 2
- Mitogen-Activated Protein Kinases
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Topics |
- Animals
- Cell Line
- Ceruletide
(pharmacology)
- Enzyme Activation
- Enzyme Inhibitors
(metabolism)
- Humans
- Janus Kinase 2
(metabolism)
- Mitogen-Activated Protein Kinases
(metabolism)
- NADPH Oxidases
(genetics, metabolism)
- Pancreas, Exocrine
(cytology, drug effects, metabolism)
- Pancreatitis
(physiopathology)
- Rats
- Reactive Oxygen Species
(metabolism)
- STAT3 Transcription Factor
(metabolism)
- Transforming Growth Factor beta1
(metabolism)
- Tyrphostins
(metabolism)
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