HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Cross-talk of the related bioactivity mediators in serum after injection of soluble TNF-α receptor on silicosis model of rats.

Abstract
This study investigates cross-talk of the related bioactivity mediators in silica-induced pulmonary inflammatory and fibrosis on rats, which contributes to the preventive and therapeutic effect of soluble TNF-α receptor. Wistar rats received saline or 50 mg of quartz by intratracheal instillation. Rats in drug-treated groups were given soluble tumor necrosis factor-α (TNF-α) receptor (500 μg) by hypodermic injection on days 1, 5 and 8 after operation. At 7 days or 14 days after instillation, rats were killed to observe the degree of injury and expression of the related bioactivity mediators including nuclear factor KB (NF-KB), nitric oxide, interleukin-1β, interleukin-10, transforming growth factor beta 1 (TGF-β1), TNF-α, interferon-Y (IFN-Y) and granulocyte macrophage colony-stimulating factor (GM-CSF). The area percentages of type I and III collagens in intervention group were lower than those in silica group. The expression of NF-κB, TGF-β1, and COL I were lower in intervention group than in silica group(p < 0.05) and GM-CSF was significantly higher (p < 0.05) at 7 days after instillation, however, NF-κB, TGF-β1, and COL I were identically lower in intervention group than in silica group, and TNF-α, IFN-γ, and GM-CSF were higher at 14 days after instillation. It may be concluded that soluble TNF-α receptor upregulating or downregulating the expression of the related bioactivity mediators results in decreasing lung injury induced by silica.
AuthorsHong-Sheng Gao, Rong Xue, Peng Ding, Nan-Fang Chen, Bing Meng, Hua-Bing Zhao, Zhen Yang, Shi-Xing Wang
JournalToxicology and industrial health (Toxicol Ind Health) Vol. 27 Issue 7 Pg. 607-16 (Aug 2011) ISSN: 1477-0393 [Electronic] England
PMID21505002 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Interleukin-1beta
  • NF-kappa B
  • Receptors, Tumor Necrosis Factor, Type I
  • Transforming Growth Factor beta1
  • Interleukin-10
  • Nitric Oxide
  • Silicon Dioxide
  • Interferon-gamma
  • Granulocyte-Macrophage Colony-Stimulating Factor
  • Collagen
Topics
  • Animals
  • Collagen (blood)
  • Disease Models, Animal
  • Enzyme-Linked Immunosorbent Assay
  • Granulocyte-Macrophage Colony-Stimulating Factor (blood)
  • Interferon-gamma (blood)
  • Interleukin-10 (blood)
  • Interleukin-1beta (blood)
  • Male
  • NF-kappa B (blood)
  • Nitric Oxide (blood)
  • Rats
  • Rats, Wistar
  • Receptors, Tumor Necrosis Factor, Type I (blood)
  • Signal Transduction
  • Silicon Dioxide (toxicity)
  • Silicosis (drug therapy)
  • Transforming Growth Factor beta1 (blood)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: