Abstract |
This study investigates cross-talk of the related bioactivity mediators in silica-induced pulmonary inflammatory and fibrosis on rats, which contributes to the preventive and therapeutic effect of soluble TNF-α receptor. Wistar rats received saline or 50 mg of quartz by intratracheal instillation. Rats in drug-treated groups were given soluble tumor necrosis factor-α (TNF-α) receptor (500 μg) by hypodermic injection on days 1, 5 and 8 after operation. At 7 days or 14 days after instillation, rats were killed to observe the degree of injury and expression of the related bioactivity mediators including nuclear factor KB ( NF-KB), nitric oxide, interleukin-1β, interleukin-10, transforming growth factor beta 1 (TGF-β1), TNF-α, interferon-Y (IFN-Y) and granulocyte macrophage colony-stimulating factor ( GM-CSF). The area percentages of type I and III collagens in intervention group were lower than those in silica group. The expression of NF-κB, TGF-β1, and COL I were lower in intervention group than in silica group(p < 0.05) and GM-CSF was significantly higher (p < 0.05) at 7 days after instillation, however, NF-κB, TGF-β1, and COL I were identically lower in intervention group than in silica group, and TNF-α, IFN-γ, and GM-CSF were higher at 14 days after instillation. It may be concluded that soluble TNF-α receptor upregulating or downregulating the expression of the related bioactivity mediators results in decreasing lung injury induced by silica.
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Authors | Hong-Sheng Gao, Rong Xue, Peng Ding, Nan-Fang Chen, Bing Meng, Hua-Bing Zhao, Zhen Yang, Shi-Xing Wang |
Journal | Toxicology and industrial health
(Toxicol Ind Health)
Vol. 27
Issue 7
Pg. 607-16
(Aug 2011)
ISSN: 1477-0393 [Electronic] England |
PMID | 21505002
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Interleukin-1beta
- NF-kappa B
- Receptors, Tumor Necrosis Factor, Type I
- Transforming Growth Factor beta1
- Interleukin-10
- Nitric Oxide
- Silicon Dioxide
- Interferon-gamma
- Granulocyte-Macrophage Colony-Stimulating Factor
- Collagen
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Topics |
- Animals
- Collagen
(blood)
- Disease Models, Animal
- Enzyme-Linked Immunosorbent Assay
- Granulocyte-Macrophage Colony-Stimulating Factor
(blood)
- Interferon-gamma
(blood)
- Interleukin-10
(blood)
- Interleukin-1beta
(blood)
- Male
- NF-kappa B
(blood)
- Nitric Oxide
(blood)
- Rats
- Rats, Wistar
- Receptors, Tumor Necrosis Factor, Type I
(blood)
- Signal Transduction
- Silicon Dioxide
(toxicity)
- Silicosis
(drug therapy)
- Transforming Growth Factor beta1
(blood)
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