Abstract | BACKGROUND: Our previous study suggests that decreased P-450(c17alpha) expression correlated with the overproduction of aldosterone in APA and nodular hyperplasia in patients with primary aldosteronism. This study was performed to further investigate if P-450(c11beta) contributes to the overproduction of aldosterone in APA and nodular hyperplasia tissues. METHODS: Total RNA and protein were extracted from 7 cases of APA tissue, 3 nodular hyperplasia tissues, 7 normal adrenal glands. P-450(c11beta) mRNA was examined by dot blot and confirmed by Northern blot analysis and by realtime PCR. Protein expression level of P-450(c11beta) was also investigated by immunohistochemical staining and confirmed by Western blot. RESULTS: The relative expression level of P-450(c11beta) mRNA to beta-actin in APA, nodular hyperplasia and the normal adrenal gland group are 47 +/- 22%, 55 +/- 13%, 64 +/- 16% respectively by dot blot and are 94 +/- 18%, 101 +/- 20%, 112 +/- 62% respectively by Northern blot. These results are further confirmed by realtime PCR. This result was also supported by the relative protein expression level of P-450(c11beta) to beta-actin which are 118 +/- 15%, 107 +/- 32%, 108 +/- 22% respectively evaluated by Western blot. There was no significant difference in protein expression level of P-450(c11beta) among the normal adrenal gland tissues, APA and adrenal nodular hyperplasia tissue, either (P > 0.05). CONCLUSIONS: These results suggest that P-450(c11beta) is not a key contributor to the overproduction of aldosterone in APA and nodular hyperplasia and can not be considered as a potential marker to differentiate between them in patients with primary aldosteronism.
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Authors | Yujiang Fang, Lei Zhao, Shuang Chen, Xijun Cui, Meifu Zang, Songsen Chen, Xu Di |
Journal | Clinical laboratory
(Clin Lab)
Vol. 57
Issue 3-4
Pg. 245-51
( 2011)
ISSN: 1433-6510 [Print] Germany |
PMID | 21500734
(Publication Type: Journal Article)
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Chemical References |
- Biomarkers
- RNA, Messenger
- Steroid 11-beta-Hydroxylase
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Topics |
- Adenoma
(physiopathology)
- Adrenal Gland Neoplasms
(physiopathology)
- Adult
- Biomarkers
(metabolism)
- Blotting, Western
- Female
- Gene Expression Regulation, Neoplastic
- Humans
- Hyperplasia
(physiopathology)
- Male
- RNA, Messenger
(metabolism)
- Reverse Transcriptase Polymerase Chain Reaction
- Steroid 11-beta-Hydroxylase
(genetics, metabolism)
- Young Adult
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