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Expression of P-450(c11beta) in adrenal aldosterone-producing adenomas and nodular hyperplasia tissues.

AbstractBACKGROUND:
Our previous study suggests that decreased P-450(c17alpha) expression correlated with the overproduction of aldosterone in APA and nodular hyperplasia in patients with primary aldosteronism. This study was performed to further investigate if P-450(c11beta) contributes to the overproduction of aldosterone in APA and nodular hyperplasia tissues.
METHODS:
Total RNA and protein were extracted from 7 cases of APA tissue, 3 nodular hyperplasia tissues, 7 normal adrenal glands. P-450(c11beta) mRNA was examined by dot blot and confirmed by Northern blot analysis and by realtime PCR. Protein expression level of P-450(c11beta) was also investigated by immunohistochemical staining and confirmed by Western blot.
RESULTS:
The relative expression level of P-450(c11beta) mRNA to beta-actin in APA, nodular hyperplasia and the normal adrenal gland group are 47 +/- 22%, 55 +/- 13%, 64 +/- 16% respectively by dot blot and are 94 +/- 18%, 101 +/- 20%, 112 +/- 62% respectively by Northern blot. These results are further confirmed by realtime PCR. This result was also supported by the relative protein expression level of P-450(c11beta) to beta-actin which are 118 +/- 15%, 107 +/- 32%, 108 +/- 22% respectively evaluated by Western blot. There was no significant difference in protein expression level of P-450(c11beta) among the normal adrenal gland tissues, APA and adrenal nodular hyperplasia tissue, either (P > 0.05).
CONCLUSIONS:
These results suggest that P-450(c11beta) is not a key contributor to the overproduction of aldosterone in APA and nodular hyperplasia and can not be considered as a potential marker to differentiate between them in patients with primary aldosteronism.
AuthorsYujiang Fang, Lei Zhao, Shuang Chen, Xijun Cui, Meifu Zang, Songsen Chen, Xu Di
JournalClinical laboratory (Clin Lab) Vol. 57 Issue 3-4 Pg. 245-51 ( 2011) ISSN: 1433-6510 [Print] Germany
PMID21500734 (Publication Type: Journal Article)
Chemical References
  • Biomarkers
  • RNA, Messenger
  • Steroid 11-beta-Hydroxylase
Topics
  • Adenoma (physiopathology)
  • Adrenal Gland Neoplasms (physiopathology)
  • Adult
  • Biomarkers (metabolism)
  • Blotting, Western
  • Female
  • Gene Expression Regulation, Neoplastic
  • Humans
  • Hyperplasia (physiopathology)
  • Male
  • RNA, Messenger (metabolism)
  • Reverse Transcriptase Polymerase Chain Reaction
  • Steroid 11-beta-Hydroxylase (genetics, metabolism)
  • Young Adult

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