Abstract | BACKGROUND: METHODS: All 45 Fisher rats were anesthetized and renal IRI model was established by 45 min clamp of bilateral renal pedicles and 24 h reperfusion. Vehicle solution or SP600125 solution was intraperitoneally injected 45 min before ischemia, respectively. Analysis of renal histology, function, reactive oxygen species (ROS) expression, JNK phosphorylation status, as well as intra-renal pro-inflammatory cytokines expression was evaluated in this study. RESULTS: After IRI, the levels of blood urea nitrogen, creatinine, tissue malondialdehyde, TNF-α, IL-1β, IL-6 were all elevated significantly, while superoxide dismutase, catalase activity were decreased. Histologic findings showed severe devastating lesions and increased rodent cell apoptosis; SP600125 effectively improved morphologic features, reversed above-mentioned parameters, and significantly attenuated c-Jun phosphorylation, as well as intra-renal pro-inflammatory cytokines expression compared with vehicle-treated group. CONCLUSION: These data demonstrate that inhibition of c-Jun with SP600125 is capable of attenuating renal IRI, which might be a novel therapy target.
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Authors | Yun-fei Xu, Min Liu, Bo Peng, Jian-ping Che, Hai-min Zhang, Yang Yan, Guang-chun Wang, Yi-chao Wu, Jun-hua Zheng |
Journal | The Journal of surgical research
(J Surg Res)
Vol. 169
Issue 1
Pg. e77-84
(Jul 2011)
ISSN: 1095-8673 [Electronic] United States |
PMID | 21492872
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2011 Elsevier Inc. All rights reserved. |
Chemical References |
- Anthracenes
- Cytokines
- Enzyme Inhibitors
- Reactive Oxygen Species
- pyrazolanthrone
- MAP Kinase Kinase 4
|
Topics |
- Animals
- Anthracenes
(pharmacology, therapeutic use)
- Apoptosis
(drug effects, physiology)
- Cytokines
(metabolism)
- Enzyme Inhibitors
(pharmacology, therapeutic use)
- Ischemia
(physiopathology)
- Kidney
(blood supply, pathology, physiopathology)
- MAP Kinase Kinase 4
(antagonists & inhibitors, drug effects, metabolism)
- Male
- Models, Animal
- Phosphorylation
- Rats
- Rats, Inbred F344
- Reactive Oxygen Species
(metabolism)
- Regional Blood Flow
(physiology)
- Reperfusion Injury
(metabolism, physiopathology, prevention & control)
- Treatment Outcome
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