Abstract | BACKGROUND: METHODS: Here we investigated the role of lumican in the development of colitis mediated by intrarectal administration of the hapten 2-4-5, trinitrobenzene sulfonic acid (TNBS) in Lum(+/+) and Lum(-/-) mice. RESULTS: The TNBS treated Lum(+/+) mouse colons showed marked increases in CXCL1, tumor necrosis factor alpha (TNF-α), and neutrophil infiltration, whereas these responses were significantly dampened in the Lum(-/-) mice. The nuclear factor kappa B (NF-κB) transcription factor, known to regulate inflammatory genes, showed a robust increase after TNBS treatment in Lum(+/+) but not in Lum(-/-) colons. Also, nuclear translocation of NF-κB was delayed in LPS stimulated Lum(-/-) primary peritoneal macrophages. CONCLUSIONS: The Lum(-/-) mice have low innate immune and inflammatory responses, but more severe body weight loss and tissue damage, a phenomenon seen in the innate immune impaired Tlr4(-/-) and MyD88(-/-) mice. Therefore, lumican promotes intestinal homeostasis by aiding innate immune and inflammatory responses that are beneficial in the early stages of colitis.
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Authors | Kristin Lohr, Hardik Sardana, Seakwoo Lee, Feng Wu, David L Huso, Abdel Rahim Hamad, Shukti Chakravarti |
Journal | Inflammatory bowel diseases
(Inflamm Bowel Dis)
Vol. 18
Issue 1
Pg. 143-51
(Jan 2012)
ISSN: 1536-4844 [Electronic] England |
PMID | 21484968
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2011 Crohn's & Colitis Foundation of America, Inc. |
Chemical References |
- Chondroitin Sulfate Proteoglycans
- Cytokines
- Haptens
- Lipopolysaccharides
- Lum protein, mouse
- Lumican
- NF-kappa B
- Toll-Like Receptor 4
- Tumor Necrosis Factor-alpha
- Trinitrobenzenesulfonic Acid
- Keratan Sulfate
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Topics |
- Animals
- Blotting, Western
- Chondroitin Sulfate Proteoglycans
(physiology)
- Colitis
(etiology, metabolism, pathology)
- Cytokines
(metabolism)
- Disease Models, Animal
- Electrophoretic Mobility Shift Assay
- Female
- Flow Cytometry
- Haptens
(toxicity)
- Inflammation
(etiology, metabolism, pathology)
- Keratan Sulfate
(physiology)
- Lipopolysaccharides
(pharmacology)
- Lumican
- Male
- Mice
- Mice, Inbred C57BL
- Mice, Knockout
- NF-kappa B
(metabolism)
- Toll-Like Receptor 4
(metabolism)
- Trinitrobenzenesulfonic Acid
(toxicity)
- Tumor Necrosis Factor-alpha
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