Cardioprotection by remote ischemic preconditioning exhibits a signaling pattern different from local ischemic preconditioning.

Abstract

Remote ischemic preconditioning (RIPC) and local ischemic preconditioning (IPC) protect the myocardium from subsequent ischemia/reperfusion (I/R) injury. In this study, the protective effects of early RIPC, IPC, and the combination of both (RIPC-IPC) were characterized. Furthermore, the hypothesis was tested that protein kinase C (PKC) and mitogen-activated protein kinases (MAPKs), important mediators of IPC, are activated in RIPC. Infarct size, serum troponin T, and creatine kinase levels were assessed after 4 × 5-min noninvasive RIPC, local IPC, or a combination of both and 35 min of regional ischemia and 120 min of reperfusion. Protein kinase C ε and the MAPKs extracellular signal-regulated MAPK (ERK), c-jun N-terminal kinase (JNK), and p38 MAPK were analyzed by Western blot analysis and activity assays in the myocardium and skeletal muscle immediately after the preconditioning protocol. Remote ischemic preconditioning, IPC, and RIPC-IPC significantly reduced myocardial infarct size (RIPC-I/R: 54% ± 15%; IPC-I/R: 33% ± 15%; RIPC-IPC-I/R: 33% ± 15%; P < 0.05 vs. I/R [76% ± 14%]) and troponin T release (RIPC-I/R: 15.4 ± 6.4 ng/mL; IPC-I/R: 10.9 ± 7.0 ng/mL; RIPC-IPC-I/R: 9.8 ± 5.6 ng/mL; P < 0.05 vs. I/R [27.1 ± 12.0 ng/mL]) after myocardial I/R. Ischemic preconditioning led to an activation of PKCε and ERK 1/2, whereas RIPC did not lead to a translocation of PKCε to the mitochondria or phosphorylation of the MAPKs ERK 1/2, JNK 1/2, and p38 MAPK. Remote ischemic preconditioning did not induce translocation of PKCε to the mitochondria or phosphorylation of MAPKs in the preconditioned muscle tissue. Remote ischemic preconditioning, IPC, and RIPC-IPC exert early protection against myocardial I/R injury. Remote ischemic preconditioning and local IPC exhibit different activation dynamics of signal transducers in the myocardium. The studied PKC-MAPK pathway is likely not involved in the protective effects of RIPC.

• Authors: Nicole M Heinen, Verena E Pütz, Jessica I Görgens, Ragnar Huhn, Yvonne Grüber, Carmen Barthuber, Benedikt Preckel, Benedikt H Pannen, Inge Bauer
• Journal: Shock (Augusta, Ga.) (Shock) Vol. 36 Issue 1 Pg. 45-53 (Jul 2011) ISSN: 1540-0514 [Electronic] United States
• PMID: 21478813 (Publication Type: Journal Article)

Chemical References

• Troponin T
• Protein Kinase C
• Extracellular Signal-Regulated MAP Kinases
• Mitogen-Activated Protein Kinases
• p38 Mitogen-Activated Protein Kinases
• Creatine Kinase

Topics

• Animals
• Blotting, Western
• Creatine Kinase (blood)
• Extracellular Signal-Regulated MAP Kinases (metabolism)
• Hemodynamics (drug effects)
• Ischemic Preconditioning
• Ischemic Preconditioning, Myocardial
• Male
• Mitogen-Activated Protein Kinases (metabolism)
• Myocardial Infarction (blood, metabolism)
• Phosphorylation
• Protein Kinase C (metabolism)
• Rats
• Rats, Wistar
• Troponin T (blood)
• p38 Mitogen-Activated Protein Kinases (metabolism)