HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Inhibition of proliferation induced by anti-sense RNA of HDAC1 in MCF-7 cells.

Abstract
Acetylation and deacetylation are the main post-translational modifications of histones. Increased deacetylation is associated with carcinogenesis, but the individual histone deacetylase (HDAC) responsible for this increase remains elusive. This study was designed to investigate the role of histone deacetylase 1 during tumorigenesis in MCF-7 human breast cancer cells. Anti-sense RNA was used to downregulate HDAC1 expression. RT-PCR analysis confirmed the efficiency of the anti-sense RNA, revealing that anti-sense RNA effectively downregulated HDAC1. The MTT assay showed that the proliferation of MCF-7 cells was inhibited and that cell activity was decreased. Cell cycle analysis identified cell cycle phase variations, with cells mainly arrested in the G1 and G2 phase. The anti-sense RNA of HDAC1 induced the inhibition of proliferation and cell cycle arrest in MCF-7 cells, suggesting it might have therapeutic applications as an antitumor agent in breast cancer.
AuthorsYibao Zhu, Qinjun Wei, Yajie Lu, Jun Yao, Xin Cao
JournalMolecular medicine reports (Mol Med Rep) 2009 Sep-Oct Vol. 2 Issue 5 Pg. 743-7 ISSN: 1791-3004 [Electronic] Greece
PMID21475895 (Publication Type: Journal Article)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: