Interfering effect and mechanism of neuregulin on experimental dementia model in rats.

Abstract

OBJECTIVE:

To investigate the effect of neuregulin 1β (NRG1β) on the neuronal apoptosis and the expressions of Bcl-2 and Bax proteins in experimental dementia model rats.

METHODS:

Thirty adult healthy male Wistar rats were randomly divided into control group, model group and treated group consisting of 10 rats, respectively. The experimental dementia models were established by injecting beta-amyloid protein 1-40 (Aβ1-40) stereotactically into the left lateral ventricle, and treated by injecting NRG1β into right lateral ventricle. The cognitive capacity of rats was evaluated with Y-electric maze. The neuronal apoptosis was counted by TUNEL assay. The expressions of Bcl-2 and Bax were determined with immunohistochemistry assay and double immunofluorescence labeling.

RESULTS:

The cognitive ability in model group rats decreased, along with the number of neuronal apoptosis and the expressions of Bcl-2 and Bax increased significantly than those in control group (P < 0.05). After treatment with NRG1β, the cognitive ability of rats improved, the number of neuronal apoptosis reduced and the expression of Bcl-2 increased while Bax decreased significantly than those in model group (P < 0.05).

CONCLUSION:

NRG1β could inhibit neuronal apoptosis by regulating the expressions of Bcl-2/Bax to improve the capacity of learning and memory in experimental dementia rats.

• Authors: Yan Chen, Meizeng Zhang, Qin Li, Yunliang Guo, Wei Ding, Lihua Wang, Zhen Zhou, Xiping Chen
• Journal: Behavioural brain research (Behav Brain Res) Vol. 222 Issue 2 Pg. 321-5 (Sep 23 2011) ISSN: 1872-7549 [Electronic] Netherlands
• PMID: 21473886 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2011 Elsevier B.V. All rights reserved.

Chemical References

• Amyloid beta-Peptides
• Bax protein, rat
• Neuregulin-1
• Neuroprotective Agents
• Peptide Fragments
• Proto-Oncogene Proteins c-bcl-2
• amyloid beta-protein (1-40)
• bcl-2-Associated X Protein
• neuregulin beta

Topics

• Amyloid beta-Peptides
• Animals
• Apoptosis (drug effects, physiology)
• Dementia (chemically induced, drug therapy, metabolism, pathology, physiopathology, psychology)
• Disease Models, Animal
• Gene Expression Regulation (drug effects, physiology)
• Hippocampus (drug effects, metabolism, pathology)
• Humans
• Injections, Intraventricular
• Male
• Maze Learning (drug effects, physiology)
• Neuregulin-1 (administration & dosage, pharmacology, physiology, therapeutic use)
• Neurons (metabolism, pathology)
• Neuroprotective Agents (administration & dosage, pharmacology, therapeutic use)
• Peptide Fragments
• Proto-Oncogene Proteins c-bcl-2 (biosynthesis)
• Rats
• Rats, Wistar
• bcl-2-Associated X Protein (biosynthesis)