Abstract |
Carriage of the natural killer (NK) receptor genotype KIR3DL1*h/*y with its HLA-B*57 ligand (*h/*y+B*57) is associated with slow time to AIDS and low viral load (VL). To provide a functional basis for these epidemiological observations, we assessed whether HIV-1-infected slow progressors (SP) carrying the *h/*y+B*57 compound genotype would have increased NK cell polyfunctional potential in comparison to SP with other killer immunoglobulin-like receptor (KIR)/HLA compound genotypes and whether this enhanced polyfunctionality was dependent upon the coexpression of both KIR3DL1*h/*y and HLA-B*57. The functional potential of NK cells was investigated by stimulating peripheral blood mononuclear cells with HLA-devoid targets or single HLA transfectants. Multiparametric flow cytometry was used to detect NK cells with seven functional profiles representing all permutations of CD107a expression and gamma interferon (IFN-γ) and tumor necrosis factor alpha (TNF-α) secretion. NK cells from individuals carrying KIR3DL1 receptor-HLA-Bw4 ligand pairs had greater trifunctional responses than those from KIR3DL1 homozygotes (hmz), who were Bw6 homozygotes. NK cells from subjects carrying the *h/*y+B*57 genotypes exhibited the highest trifunctional potential, and this was dependent on cocarriage of the NK receptor and its ligand. Trifunctional cells secreted more of each function tested on a per-cell basis than each corresponding monofunctional NK subset. Although VL influenced NK functionality, individuals with defined KIR/HLA genotypes exhibited differences in NK cell polyfunctionality that could not be accounted for by VL alone. The protective effect of HLA-B*57 on slow progression to AIDS and low VL may be mediated through its interaction with KIR3DL1 alleles to educate NK cells for potent activity upon stimulation.
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Authors | Philomena Kamya, Salix Boulet, Christos M Tsoukas, Jean-Pierre Routy, Réjean Thomas, Pierre Côté, Mohamed-Rachid Boulassel, Jean-Guy Baril, Colin Kovacs, Stephen A Migueles, Mark Connors, Todd J Suscovich, Christian Brander, Cecile L Tremblay, Nicole Bernard, Canadian Cohort of HIV Infected Slow Progressors |
Journal | Journal of virology
(J Virol)
Vol. 85
Issue 12
Pg. 5949-60
(Jun 2011)
ISSN: 1098-5514 [Electronic] United States |
PMID | 21471235
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- HLA-B Antigens
- HLA-B57 antigen
- KIR3DL1 protein, human
- Ligands
- Receptors, KIR3DL1
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Topics |
- Adult
- Aged
- Female
- HIV Infections
(immunology, virology)
- HIV Long-Term Survivors
- HIV-1
(immunology)
- HLA-B Antigens
(genetics, metabolism)
- Humans
- Killer Cells, Natural
(immunology)
- Ligands
- Male
- Middle Aged
- Receptors, KIR3DL1
(genetics, metabolism)
- Young Adult
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