Distinct proteinase 3-induced cytokine patterns in Wegener´s granulomatosis, Churg-Strauss syndrome, and healthy controls.

Abstract

OBJECTIVES:

To analyse whether a specific cytokine pattern is elicited in response to the autoantigen proteinase 3 (PR3) in active Wegener's granulomatosis (WG).

METHODS:

Six-colour flow cytometry was used to analyse cytokine production and surface markers of the total CD4+ T-cell population ex vivo and in PR3-stimulated T-cell lines of patients with active PR3-ANCA-positive WG, PR3-ANCA-negative Churg-Strauss syndrome (CSS), and healthy controls (HC).

RESULTS:

The cytokine response of the total PB CD4+ T cell population was skewed towards distinct pro-inflammatory cytokine patterns in WG (Th1-type) and CSS (Th17, Th1-/Th2-type). Th2-type as well as Th17 cell populations including Th17/Th1, Th17/Th2 and Th22 cells were elicited in response to PR3 stimulation in WG. In contrast, CSS patients displayed a Th2-type dominated response following PR3 stimulation.

CONCLUSIONS:

These data suggest that the cytokine response of the total CD4+ T-cell population and PR3-specific cells is influenced by the underlying disorder.

• Authors: Ursula Fagin, Elena Csernok, Antje Müller, Silke Pitann, Juliane Fazio, Kristina Krause, Philip Bremer, Edith Wipfler-Freissmuth, Frank Moosig, Wolfgang L Gross, Peter Lamprecht
• Journal: Clinical and experimental rheumatology (Clin Exp Rheumatol) 2011 Jan-Feb Vol. 29 Issue 1 Suppl 64 Pg. S57-62 ISSN: 0392-856X [Print] Italy
• PMID: 21470489 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antibodies, Antineutrophil Cytoplasmic
• Autoantigens
• Cytokines
• Inflammation Mediators
• Myeloblastin

Topics

• Adult
• Aged
• Aged, 80 and over
• Antibodies, Antineutrophil Cytoplasmic (blood)
• Autoantigens
• Case-Control Studies
• Cell Line
• Churg-Strauss Syndrome (enzymology, immunology)
• Cytokines (metabolism)
• Female
• Flow Cytometry
• Germany
• Granulomatosis with Polyangiitis (enzymology, immunology)
• Humans
• Inflammation Mediators (metabolism)
• Male
• Middle Aged
• Myeloblastin (immunology)
• T-Lymphocytes, Helper-Inducer (enzymology, immunology)
• Th1 Cells (enzymology, immunology)
• Th17 Cells (enzymology, immunology)
• Th2 Cells (enzymology, immunology)
• Up-Regulation
• Young Adult