Abstract |
2-isoprenylhydroquinone-1-glucoside (1), 3,5-dicaffeoylquinic acid (2), and 3,5-dicaffeoylquinic acid methyl ester (3), isolated from Phagnalon rupestre, improved the contact hypersensitivity response to 2,4,6-trinitrochlorobenzene in mice. These phenolics reduced ear swelling and IL-1β content by 50% 24 h after challenge; in addition, 2 inhibited tumor necrosis factor-α by 53%. All three compounds also reduced interleukin-2 content by 50% 72 h after challenge. Both 2 and 3 inhibited metalloproteinase-9 levels in the skin lesions by 66% and 41%, respectively, and lowered cyclooxygenase-2 expression by 44% and 49%, respectively, at 24 h. Moreover, 2 was effective against atopic dermatitis induced by repeated application of 2,4,6- trinitrochlorobenzene; it attenuated edema by over 40% from day 7 and inhibited inflammatory cell infiltration by 44% at day 22. In addition, 1-3 reduced metalloproteinase-9 expression in a dose-dependent manner in macrophages RAW 264.7 stimulated with lipopolysaccharide. Thus, compounds 2 and 3 were found to exhibit a greater activity against contact hypersensitivity than 1.
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Authors | Elisa Giner, Mariya El Alami, Salvador Máñez, M Carmen Recio, José-Luis Ríos, Rosa M Giner |
Journal | Journal of natural products
(J Nat Prod)
Vol. 74
Issue 5
Pg. 1079-84
(May 27 2011)
ISSN: 1520-6025 [Electronic] United States |
PMID | 21469692
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Cyclooxygenase 2 Inhibitors
- Interleukin-1beta
- Interleukin-2
- Lipopolysaccharides
- Phenols
- Tumor Necrosis Factor-alpha
- Picryl Chloride
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Topics |
- Animals
- Cyclooxygenase 2 Inhibitors
(pharmacology)
- Dermatitis, Contact
(pathology)
- Disease Models, Animal
- Dose-Response Relationship, Drug
- Ear
(pathology)
- Edema
(chemically induced)
- Interleukin-1beta
(analysis, metabolism)
- Interleukin-2
(analysis)
- Lipopolysaccharides
(pharmacology)
- Macrophages
(drug effects)
- Mice
- Phenols
(chemistry, isolation & purification, pharmacology)
- Picryl Chloride
(pharmacology)
- Skin
(drug effects)
- Tumor Necrosis Factor-alpha
(analysis, metabolism)
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