Abstract | BACKGROUND: METHODS: PCA cell lines LNCaP, C4-2B, and 22RV1 were exposed to LH. Gene expression was quantified using real-time PCR and protein expression was characterized with standard Western blot analysis. Steroid analysis was performed using radioimmunoassay (RIA). Cell viability was measured using an MTS viability assay. RESULTS:
Androgen-sensitive (LNCaP) and -independent PCA cells (C4-2B and 22RV1) express both mRNA and protein for LH and LH receptor (LHR). Exposure of these cells to LH for 4 hr increased the expression of several steroidogenic genes. Exposure for 10 days resulted in the increase of additional genes. At both time points, the upregulation of these genes was dose-dependent. This was mirrored by an increase in the expression of several key steroidogenic enzymes, including StAR, CYB5B, CYP11A, and 3βHSD. LH stimulated the production of progesterone and testosterone in LNCaP cells as measured by RIA. We have also demonstrated that treatment of LNCaP cells with LH enhanced their viability. CONCLUSIONS: Our data show that LH-mediated activation of LHR significantly up-regulates the expression of genes and enzymes required for steroidogenesis and increases steroid production in PCA cells.
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Authors | Jacek Pinski, Shigang Xiong, Qingcai Wang, Frank Stanczyk, Debra Hawes, Stephen V Liu |
Journal | The Prostate
(Prostate)
Vol. 71
Issue 8
Pg. 892-8
(Jun 01 2011)
ISSN: 1097-0045 [Electronic] United States |
PMID | 21456071
(Publication Type: Journal Article)
|
Copyright | Copyright © 2010 Wiley-Liss, Inc. |
Chemical References |
- Receptors, LH
- Testosterone
- Progesterone
- Luteinizing Hormone
|
Topics |
- Carcinoma
(metabolism)
- Cell Line, Tumor
- Gene Expression Profiling
- Gene Expression Regulation, Neoplastic
(drug effects)
- Humans
- Luteinizing Hormone
(pharmacology)
- Male
- Progesterone
(biosynthesis)
- Prostatic Neoplasms
(metabolism)
- Receptors, LH
(biosynthesis)
- Testosterone
(biosynthesis)
- Up-Regulation
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