Targeted photodynamic therapy of breast cancer cells using antibody-phthalocyanine-gold nanoparticle conjugates.

Abstract

A 4-component antibody-phthalocyanine-polyethylene glycol-gold nanoparticle conjugate is described for use as a potential drug for targeted photodynamic cancer therapy. Gold nanoparticles (4 nm) were stabilised with a self-assembled layer of a zinc-phthalocyanine derivative (photosensitiser) and a heterobifunctional polyethylene glycol. Anti-HER2 monoclonal antibodies were covalently bound to the nanoparticles via a terminal carboxy moiety on the polyethylene glycol. The nanoparticle conjugates were stable towards aggregation, and under irradiation with visible red light efficiently produced cytotoxic singlet oxygen. Cellular experiments demonstrated that the nanoparticle conjugates selectively target breast cancer cells that overexpress the HER2 epidermal growth factor cell surface receptor, and that they are effective photodynamic therapy agents.

• Authors: Tanya Stuchinskaya, Miguel Moreno, Michael J Cook, Dylan R Edwards, David A Russell
• Journal: Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology (Photochem Photobiol Sci) Vol. 10 Issue 5 Pg. 822-31 (May 2011) ISSN: 1474-9092 [Electronic] England
• PMID: 21455532 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antibodies, Monoclonal
• Indoles
• Isoindoles
• Photosensitizing Agents
• Singlet Oxygen
• Polyethylene Glycols
• Gold
• Receptor, ErbB-2
• phthalocyanine

Topics

• Antibodies, Monoclonal (chemistry, immunology)
• Breast Neoplasms (drug therapy)
• Cell Line, Tumor
• Female
• Gold (chemistry)
• Humans
• Indoles (chemistry)
• Isoindoles
• Metal Nanoparticles (chemistry)
• Photochemotherapy
• Photosensitizing Agents (chemistry, therapeutic use, toxicity)
• Polyethylene Glycols (chemistry)
• Receptor, ErbB-2 (immunology)
• Singlet Oxygen (metabolism)