Abstract |
Lentiviral vectors (LVs) pseudotyped with envelope proteins of alphaviruses have recently attracted considerable interest for their potential as gene delivery tools. We report the production of human immunodeficiency virus type 1 (HIV-1)-derived LVs pseudotyped with envelope glycoproteins derived from the Aura virus ( AURA). We found that the AURA- glycoprotein-pseudotyped LVs use C-type lectins ( DC-SIGN and L-SIGN) as attachment factors. These interactions with DC-SIGN are specific as determined by inhibition assays and appear to facilitate transduction through a pH-dependent pathway. AURA-pseudotyped LVs were used to transduce monocyte-derived dendritic cells (DCs) and the transduction was shown to be DC-SIGN mediated, as illustrated by competitive inhibition with DC-SIGN and L-SIGN antibodies and yeast mannan. Comparisons with LVs enveloped with glycoproteins derived from vesicular stomatitis virus and Sindbis virus suggest that AURA- glycoprotein-bearing LVs might be useful to genetically modify DCs for the study of DC biology and DC-based immunotherapy.
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Authors | Steven Froelich, April Tai, Katie Kennedy, Adnan Zubair, Pin Wang |
Journal | Human gene therapy
(Hum Gene Ther)
Vol. 22
Issue 10
Pg. 1281-91
(Oct 2011)
ISSN: 1557-7422 [Electronic] United States |
PMID | 21452926
(Publication Type: Journal Article, Research Support, N.I.H., Extramural)
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Chemical References |
- Cell Adhesion Molecules
- DC-specific ICAM-3 grabbing nonintegrin
- Lectins, C-Type
- Receptors, Cell Surface
- Viral Envelope Proteins
- Green Fluorescent Proteins
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Topics |
- Alphavirus
(metabolism)
- Animals
- Cell Adhesion Molecules
(genetics, metabolism)
- Dendritic Cells
(metabolism)
- Gene Transfer Techniques
- Genes, vpr
(genetics)
- Genetic Engineering
(methods)
- Genetic Vectors
(biosynthesis, genetics)
- Green Fluorescent Proteins
(genetics, metabolism)
- HEK293 Cells
- HIV-1
(genetics)
- Humans
- Lectins, C-Type
(genetics, metabolism)
- Mice
- Microscopy, Confocal
- NIH 3T3 Cells
- Plasmids
(genetics)
- Receptors, Cell Surface
(genetics, metabolism)
- Transduction, Genetic
(methods)
- Viral Envelope Proteins
(metabolism)
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