[Study total glucosides of Radix Paeoniae Rubra induced K562 tumor cell apoptosis of signaling pathways and related gene changes].

Abstract

OBJECTIVE:

To study the total glucosides of Radix Paeoniae Rubra induced K562 tumor cell apoptosis of signaling pathways and related gene changes.

METHOD:

By MTT and flow cytometric, real-time quantitatie polymerase chain reaction (PCR) and Western blot methods researched the level of genes and proteins.

RESULT:

The total glucosides of Radix Paeoniae Rubra could inhibit K562 cell growth by MTT method, there was the relationship of concentration-time between them, TGC prompted K562 cell translocation of phosphatidylserine, may be apoptosis through non-receptor-dependent pathways because caspase-3 mRNA, caspase-9 mRNA and cytochrome C increased, caspase-8 mRNA had no significant change. The expression of Bcl-2 protein and Bcl-X1 protein could decreased, the expression of Bax protein could increased by regulating gene expression.

CONCLUSION:

TGC induced K562 cell apoptosis that might be through the mitochondria pathway, when cell apoptosis occured, Bcl-2 or Bcl-XI proteins combination of Bax protein would be displacement, then the mitochondrial membrane became permeable to release a series of material, then lead to cell death.

• Authors: Huiyu Xu, Zhiwei Chen, Li Zhou, Jianzhao Niu
• Journal: Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica (Zhongguo Zhong Yao Za Zhi) Vol. 35 Issue 24 Pg. 3377-81 (Dec 2010) ISSN: 1001-5302 [Print] China
• PMID: 21438411 (Publication Type: English Abstract, Journal Article)

Chemical References

• Antineoplastic Agents
• Glucosides
• Caspases

Topics

• Antineoplastic Agents (pharmacology)
• Apoptosis (drug effects, genetics)
• Caspases (genetics)
• Dose-Response Relationship, Drug
• Gene Expression Regulation, Neoplastic (drug effects)
• Glucosides (pharmacology)
• Humans
• K562 Cells
• Paeonia (chemistry)
• Signal Transduction (drug effects)
• Time Factors