[Protection and mechanisms of salvianolic-acid B on experimental renal interstitial fibrosis in rats].

Abstract

OBJECTIVE:

To investigate the effect and mechanism of salvianolic acid B (SA-B) on renal interstitial fibrosis in rats induced by unilateral ureteral obstruction (UUO).

METHODS:

18 male SD rats were randomly divided into 3 groups, 6 in each group. After the models were established, the rats were treated with SA-B for 2 weeks. Then their renal pathology were examined by hight microscope and electron microscopy Protein expression levels of alpha-smooth muscle actin (alpha-SMA) and E-cadherin (E-cad) in the obstructed kidney were analyzed by Western blot and Biochemistry assay.

RESULTS:

Pathological examination of the kidney in model group showed tubules lumen widened and many inflammatory cells infiltrated, a part of renal tubule expanded and part of them atrophied. The tubular epithelial cells were karyorrhexis or karyolysis, some tubulars were atrophy. The protein expression of alpha-SMA were significantly up-regulated (P < 0.01) and E-cad were significantly down-regulated (P < 0.01) in the model group. After intervention with SA-B, the renal pathological status in the treatment group was significantly improved, the expression of alpha-SMA were significantly down-regulated (P < 0.05), but E-cad only a little up-regulated (P > 0.05).

CONCLUSION:

SA-B could antagonize renal interstitial fibrosis mainly by maintaining epithelial phenotype, inhibiting the protein of alpha-SMA which is the principal effect cells that are responsible for the progressive kidney fibrosis.

• Authors: Hai-Ying Lu, Juan Zhou, Min Lu, Yu-Min Liu, Fei Wang, Min Lin, Yue Zhang
• Journal: Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials (Zhong Yao Cai) Vol. 33 Issue 11 Pg. 1755-9 (Nov 2010) ISSN: 1001-4454 [Print] China
• PMID: 21434439 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Actins
• Benzofurans
• Cadherins
• salvianolic acid B

Topics

• Actins (metabolism)
• Animals
• Benzofurans (pharmacology, therapeutic use)
• Blotting, Western
• Cadherins (metabolism)
• Disease Models, Animal
• Fibrosis (drug therapy, pathology)
• Immunohistochemistry
• Kidney (metabolism, pathology)
• Kidney Diseases (drug therapy, etiology, pathology)
• Kidney Tubules (metabolism, pathology)
• Male
• Random Allocation
• Rats
• Rats, Sprague-Dawley
• Ureteral Obstruction (complications)