Abstract | PURPOSE: MATERIALS AND METHODS: Enrolled in this study were 66 patients and 82 healthy volunteers. From the first 14 patients with bladder cancer we obtained samples of malignant and normal bladder tissue. All patients and volunteers provided a urine sample. Protein extracts of tissue specimens were separated by 2-dimensional gel electrophoresis for comparative analysis of neoplastic and normal tissue. Phosphoproteins were studied by Western blot and characterized by mass spectrometry. Urine samples were analyzed by 1-dimensional gel electrophoresis. Phosphoproteins were measured by affinity dot blotting. RESULTS: Profound changes in the pattern of protein tyrosine phosphorylation were consistently, reproducibly observed in bladder cancer tissues. A total of 24 phosphorylated proteins were differentially expressed in cancer tissue and identified by mass spectrometry. Phosphoproteins were fairly stable in urine samples, leading to accumulation. Urinary tyrosine phosphoproteins showed the most remarkable changes in patients with cancer with an approximately 5-fold increase compared to levels in healthy controls. CONCLUSIONS:
|
Authors | Amina Khadjavi, Giovanna Barbero, Paolo Destefanis, Giorgia Mandili, Giuliana Giribaldi, Franca Mannu, Antonella Pantaleo, Carlo Ceruti, Andrea Bosio, Luigi Rolle, Francesco Turrini, Dario Fontana |
Journal | The Journal of urology
(J Urol)
Vol. 185
Issue 5
Pg. 1922-9
(May 2011)
ISSN: 1527-3792 [Electronic] United States |
PMID | 21421240
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
|
Copyright | Copyright © 2011 American Urological Association Education and Research, Inc. Published by Elsevier Inc. All rights reserved. |
Chemical References |
- Biomarkers, Tumor
- Tyrosine
|
Topics |
- Aged
- Biomarkers, Tumor
(urine)
- Biopsy
- Blotting, Western
- Case-Control Studies
- Electrophoresis, Agar Gel
- Female
- Humans
- Immunoblotting
- Male
- Mass Spectrometry
- Neoplasm Staging
- Phosphorylation
- ROC Curve
- Tyrosine
(urine)
- Urinary Bladder Neoplasms
(diagnosis, pathology, surgery, urine)
|