Behavioral and physiological effects of RDX on adult zebrafish.

Abstract

1,3,5-Trinitroperhydro-1,3,5-triazine (RDX) is a nitroamine explosive, with common toxic effects including seizures. Here, we explore the behavioral effects of acute RDX exposure in adult zebrafish Danio rerio, a rapidly developing model in neuroscience and neurotoxicology research. Overall, a 30-min exposure to RDX low dose of 0.1 mM evoked behavioral activation in zebrafish, while a higher dose of 1 mM markedly reduced exploration, increased freezing and evoked seizure-like responses (i.e., bouts of hyperactivity, spasms, and corkscrew swimming). Likewise, whole-body cortisol levels were also significantly elevated in fish exposed to 1 mM (but not 0.1 mM) RDX. In line with clinical and animal data, our study demonstrates the dose-dependent behavioral activation and pro-convulsant effects of RDX in zebrafish-based models.

• Authors: Larry R Williams, Keith Wong, Adam Stewart, Christopher Suciu, Siddharth Gaikwad, Nadine Wu, John Dileo, Leah Grossman, Jonathan Cachat, Peter Hart, Allan V Kalueff
• Journal: Comparative biochemistry and physiology. Toxicology & pharmacology : CBP (Comp Biochem Physiol C Toxicol Pharmacol) Vol. 155 Issue 1 Pg. 33-8 (Jan 2012) ISSN: 1532-0456 [Print] United States
• PMID: 21382508 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2011 Elsevier Inc. All rights reserved.

Chemical References

• Triazines
• cyclonite
• Hydrocortisone

Topics

• Animals
• Behavior, Animal (drug effects)
• Female
• Hydrocortisone (analysis)
• Hyperkinesis (chemically induced)
• Locomotion (drug effects)
• Male
• Seizures (chemically induced)
• Spasm (chemically induced)
• Swimming
• Time Factors
• Toxicity Tests, Acute
• Triazines (toxicity)
• Zebrafish (physiology)