HOMEPRODUCTSSERVICESCOMPANYCONTACTFAQResearchDictionaryPharmaMobileSign Up FREE or Login

DNA-methylation profiling distinguishes malignant melanomas from benign nevi.

Abstract
DNA methylation, an epigenetic alteration typically occurring early in cancer development, could aid in the molecular diagnosis of melanoma. We determined technical feasibility for high-throughput DNA-methylation array-based profiling using formalin-fixed paraffin-embedded tissues for selection of candidate DNA-methylation differences between melanomas and nevi. Promoter methylation was evaluated in 27 common benign nevi and 22 primary invasive melanomas using a 1505 CpG site microarray. Unsupervised hierarchical clustering distinguished melanomas from nevi; 26 CpG sites in 22 genes were identified with significantly different methylation levels between melanomas and nevi after adjustment for age, sex, and multiple comparisons and with β-value differences of ≥ 0.2. Prediction analysis for microarrays identified 12 CpG loci that were highly predictive of melanoma, with area under the receiver operating characteristic curves of > 0.95. Of our panel of 22 genes, 14 were statistically significant in an independent sample set of 29 nevi (including dysplastic nevi) and 25 primary invasive melanomas after adjustment for age, sex, and multiple comparisons. This first report of a DNA-methylation signature discriminating melanomas from nevi indicates that DNA methylation appears promising as an additional tool for enhancing melanoma diagnosis.
AuthorsKathleen Conway, Sharon N Edmiston, Zakaria S Khondker, Pamela A Groben, Xin Zhou, Haitao Chu, Pei Fen Kuan, Honglin Hao, Craig Carson, Marianne Berwick, David W Olilla, Nancy E Thomas
JournalPigment cell & melanoma research (Pigment Cell Melanoma Res) Vol. 24 Issue 2 Pg. 352-60 (Apr 2011) ISSN: 1755-148X [Electronic] England
PMID21375697 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
Copyright© 2011 John Wiley & Sons A/S.
Chemical References
  • Biomarkers, Tumor
Topics
  • Biomarkers, Tumor (genetics)
  • DNA Methylation
  • Epigenesis, Genetic
  • Gene Expression Profiling
  • Humans
  • Melanoma (diagnosis, genetics, pathology)
  • Nevus (diagnosis, genetics, pathology)
  • ROC Curve
  • Skin Neoplasms (diagnosis, genetics, pathology)
  • Tissue Array Analysis

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research network!


Choose Username:
Email:
Password:
Verify Password: