Abstract | BACKGROUND: AIM: METHODS: The study comprised 33 individuals; 25 with collagenous colitis (14 in clinical remission without treatment, 11 with active disease and 10 examined in clinical remission resulting from treatment with 6 mg budesonide); eight healthy individuals undergoing screening colonoscopy served as controls. Endoscopic biopsies from the sigmoid colon were mounted in modified Ussing chambers and assessed for short-circuit current (Isc), potential difference, trans-epithelial resistance and transmucosal passage of Escherichia coli K12 after adding 100 μmol/L CDCA or DCA. RESULTS: When adding 100 μmol/L CDCA or DCA, bacterial uptake increased fourfold in biopsies of patients in remission; CDCA 6.5 units [2.5-9.8] and DCA 6.2 units [2.1-22] (median [IQR]), compared with uptake in biopsies without added bile acids 1.6 units [1.1-3] (P=0.004 and P=0.01 respectively). In active disease and in patients in remission due to budesonide treatment, bile acids did not affect bacterial uptake. Confocal microscopy revealed trans-epithelial passage of E. coli K12 within 30 min. CONCLUSIONS: Low concentrations of dihydroxy- bile acids exacerbate mucosal barrier dysfunction in colonic biopsies of patients with collagenous colitis in remission. This allows a substantially increased bacterial uptake, which may contribute to recurrence of inflammation.
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Authors | A Münch, J D Söderholm, A Ost, A H Carlsson, K-E Magnusson, M Ström |
Journal | Alimentary pharmacology & therapeutics
(Aliment Pharmacol Ther)
Vol. 33
Issue 8
Pg. 954-60
(Apr 2011)
ISSN: 1365-2036 [Electronic] England |
PMID | 21366635
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | © 2011 Blackwell Publishing Ltd. |
Chemical References |
- Bile Acids and Salts
- Gastrointestinal Agents
- Deoxycholic Acid
- Chenodeoxycholic Acid
- Budesonide
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Topics |
- Adult
- Aged
- Aged, 80 and over
- Bile Acids and Salts
(pharmacology)
- Biological Transport
- Biopsy
- Budesonide
(therapeutic use)
- Case-Control Studies
- Chenodeoxycholic Acid
(pharmacology)
- Colitis, Collagenous
(metabolism, microbiology, pathology)
- Deoxycholic Acid
(pharmacology)
- Escherichia coli K12
(metabolism)
- Female
- Gastrointestinal Agents
(administration & dosage)
- Humans
- In Vitro Techniques
- Male
- Microscopy, Confocal
- Middle Aged
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