Artemisinin evaluation in Romanian Artemisia annua wild plants using a new HPLC/MS method.

Artemisinin, a sesquiterpene lactone from Artemisia annua L., has received considerable attention in the last few decades as a potent antimalarial drug. Artemisinin has rather low toxicity; it is effective against drug-resistant Plasmodium species and against cerebral malaria. This study reports the development of a rapid and sensitive assay for the quantification of artemisinin in A. annua by reversed phase HPLC/MS. In the selected optimal experimental conditions, artemisinin exhibited a well-defined chromatographic peak with a retention time of 2 ± 0.2 min. The chromatographic signal shows a linear dependence with artemisinin concentration, enabling the use of this signal for artemisinin quantification according to the following regression equation: y = 2665.40x - 14697.61. The correlation coefficient (R(2)) was 0.9989. For every concentration within the range of the standard curve (0.1-2 µg mL(-1)), accuracy was between 95 and 104%. Artemisinin content in Romanian A. annua wild plants varies between 0.17 and 0.21% (dry weight basis).
AuthorsB Ivanescu, L Vlase, A Corciova, M I Lazar
JournalNatural product research (Nat Prod Res) Vol. 25 Issue 7 Pg. 716-22 (Apr 2011) ISSN: 1478-6427 [Electronic] England
PMID21360406 (Publication Type: Journal Article)
Chemical References
  • Antimalarials
  • Artemisinins
  • Plant Extracts
  • artemisinine
  • Antimalarials (analysis)
  • Artemisia annua (chemistry)
  • Artemisinins (analysis)
  • Chromatography, High Pressure Liquid (methods)
  • Plant Components, Aerial (chemistry)
  • Plant Extracts (chemistry)
  • Romania
  • Tandem Mass Spectrometry

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research network!

Choose Username:
Verify Password: