Human
cervical cancer cell lines (HeLa cells) were incubated with
serum-free media for 24 hours. These cells were then treated with
Verapamil for different time points. Cell proliferation was evaluated by MTT assay and EdU fluorescent assay. The migration of cells was measured by the wound healing assay. Results MTT assay demonstrated that,
Verapamil (1, 5, 10 and 20 microg/mL concentrations) could inhibit the proliferation of HeLa cells after 48 or 72 hours treatment. The inhibition rate for 48 h-treatment was 20.87% +/- 1.71%, 23.55% +/- 4.46%, 28.65% +/- 1.32%, 27.37% +/- 3.05% respectively (P < 0.05). After 5, 10 and 20 microg/mL concentrations of
verapamil treatment for 24h, the proportion of HeLa cells in the stage of
DNA synthesis was 15.7% +/- 1.2%, 11.7% +/- 0.3%, 2.8% +/- 0.5% as evaluated with EdU fluorescent assay, which was significantly lower than that of control (27.34% +/- 2.1%, P < 0.05). With the increase of
drug concentrations,
Verapamil could significantly suppressed the
DNA synthesis in HeLa cell. Cell migration assay revealed that, treatment with
Verapamil (5, 10 and 20 microg/mL) for 24 hours significantly inhibited cells migration distance (P < 0.05).
CONCLUSION: