[Effects of the combination of sandostatin and gardenia jasminoides ellis on pancreatic mitochondria injury in severe acute pancreatitis rats].

Abstract

OBJECTIVE:

To study the protective effects of the combination of sandostatin(SS) and gardenia jasminoides ellis (GJE) on pancreatic mitochondria injury in severe acute pancreatitis (SAP) rats.

METHODS:

SAP rat model was induced by retrograde injection of 15 g/L sodium deoxycholate into biliary tract. The rats were randomly divided into five groups: SAP group, Sham group, SS group, GJE group, as well as SS and GJE combination group. The changes of Superoxide dismutase(SOD), succinic dehydrogenase (SDH), Na+ -K+ -ATPase, Ca2+ - Mg2+ -ATPase, malondialdehyde (MDA), membrance fluidity of mitochondria in pancreatic tissue were measured.

RESULTS:

Compared to Sham group, the activities of SOD, SDH, Na+ -K+ -ATPase and Ca2+ -Mg2+ -ATPase in mitochondria were obviously decreased in SAP group, and MDA and microviscosity (eta) were markedly increased (P < 0.01), which were associated with the increase of serum amylase level (P < 0.01). The above-mentioned indexes of GJE group, SS group and combination group were obviously ameliorated in comparison with SAP group (P < 0.01), the improvement in combination group was the best (P < 0.05 or P < 0.01). The correlation analysis indicated that MDA was negatively correlated with SOD, membrance fluidity, as well as the activities of Na+ -K+ -ATPase and Ca2+ -Mg2- -ATPase (r = -0.857, P < 0.01; r = -0.960, P < 0.01; r = -0.966, P < 0.01; r = -0.926, P < 0.01).

CONCLUSION:

The combination of sandostatin and gardenia jasminoides ellis can protect pancreatic mitochondria injury in severe acute pancreatitis.

• Authors: Yan-lei Wang, Jian-yu Liu, You-ling Jing, Yi-bing Zhang, Sun-Na, Xiao-jun Wang
• Journal: Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition (Sichuan Da Xue Xue Bao Yi Xue Ban) Vol. 42 Issue 1 Pg. 37-40 (Jan 2011) ISSN: 1672-173X [Print] China
• PMID: 21355297 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antioxidants
• Drugs, Chinese Herbal
• Protective Agents
• Superoxide Dismutase
• Octreotide

Topics

• Animals
• Antioxidants (therapeutic use)
• Drug Therapy, Combination
• Drugs, Chinese Herbal (therapeutic use)
• Female
• Gardenia (chemistry)
• Male
• Mitochondria (drug effects, metabolism, pathology)
• Octreotide (therapeutic use)
• Pancreatitis, Acute Necrotizing (drug therapy)
• Protective Agents (therapeutic use)
• Random Allocation
• Rats
• Rats, Sprague-Dawley
• Superoxide Dismutase (metabolism)