Protection against 1-methyl-4-phenylpyridinium ion (MPP+)-induced apoptosis by water extract of ginseng (Panax ginseng C.A. Meyer) in SH-SY5Y cells.

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE:

The present study investigates the protective effects of water extract of ginseng (Panax ginseng C.A. Meyer) against 1-methyl-4-phenylpyridinium ion (MPP(+))-induced cytotoxicity in SH-SY5Y human neuroblastoma cells and explores the underlying mechanisms. The approach may be used for screening therapeutic agents for degenerative disorders such as Parkinson's disease.

MATERIALS AND METHODS:

SH-SY5Y human neuroblastoma cells were used to analyze the protective effects of water extract of ginseng (WEG) against multiple parameters such as MPP(+)-induced viability, oxidative injury, expression of Bax, Bcl-2, cytochrome c and cleaved caspase-3.

RESULTS:

WEG exerted inhibitory effect on cell death, overproduction of ROS, elevated Bax/Bcl-2 ratio, release of cytochrome c and activation of caspase-3 expression in MPP(+)-treated SH-SY5Y cells.

CONCLUSIONS:

WEG exhibited significant protective effects against MPP(+)-induced cytotoxicity in SH-SY5Y cells possibly through the suppression of ROS generation and the inhibition of mitochondria-dependent apoptotic pathway.

• Authors: Shengquan Hu, Renwen Han, Shinghung Mak, Yifan Han
• Journal: Journal of ethnopharmacology (J Ethnopharmacol) Vol. 135 Issue 1 Pg. 34-42 (Apr 26 2011) ISSN: 1872-7573 [Electronic] Ireland
• PMID: 21349320 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2011 Elsevier Ireland Ltd. All rights reserved.

Chemical References

• Antioxidants
• Enzyme Inhibitors
• Herbicides
• Neurotoxins
• Plant Extracts
• Proto-Oncogene Proteins c-bcl-2
• Reactive Oxygen Species
• bcl-2-Associated X Protein
• Cytochromes c
• Caspase 3
• 1-Methyl-4-phenylpyridinium

Topics

• 1-Methyl-4-phenylpyridinium
• Antioxidants (pharmacology, therapeutic use)
• Apoptosis (drug effects)
• Caspase 3 (metabolism)
• Cell Line, Tumor
• Cytochromes c (metabolism)
• Drug Evaluation, Preclinical
• Enzyme Inhibitors (pharmacology, therapeutic use)
• Herbicides
• Humans
• Mitochondria (drug effects, metabolism)
• Neuroblastoma (metabolism)
• Neurotoxins
• Panax
• Parkinson Disease (drug therapy, metabolism)
• Phytotherapy
• Plant Extracts (pharmacology, therapeutic use)
• Proto-Oncogene Proteins c-bcl-2 (metabolism)
• Reactive Oxygen Species (metabolism)
• bcl-2-Associated X Protein (metabolism)