Avian and human influenza A virus receptors in trachea and lung of animals.

Abstract

BACKGROUND:

Influenza A viruses are capable of crossing the specific barrier between human beings and animals resulting in interspecies transmission. The important factor of potential infectivity of influenza A viruses is the suitability of the receptor binding site of the host and viruses. The affinities of avian and human influenza virus to bind with the receptors and the distributions of receptors in animals are different.

OBJECTIVE:

This study aims to investigate the anatomical distribution of avian and human influenza virus receptors using the double staining lectin histochemistry method.

METHODS:

Double staining of lectin histochemistry was performed to identify both SA alpha2,3 Gal and SA alpha2,6 Gal receptors in trachea and lung tissue of dogs, cats, tigers, ferret, pigs, ducks and chickens.

RESULTS:

We have demonstrated that avian and human influenza virus receptors were abundantly present in trachea, bronchus and bronchiole, but in alveoli of dogs, cats and tigers showed SA alpha2,6 Gal only. Furthermore, endothelial cells in lung tissues showed presence of SA alpha2,3 Gal.

CONCLUSION:

The positive sites of both receptors in respiratory tract, especially in the trachea, suggest that all mammalian species studied can be infected with avian influenza virus. These findings suggested that dogs and cats in close contact with humans should be of greater concern as an intermediate host for avian influenza A in which there is the potential for viral adaptation and reassortment.

• Authors: Sukanya Thongratsakul, Yasuo Suzuki, Hiroaki Hiramatsu, Thavajchai Sakpuaram, Theerapol Sirinarumitr, Chaithep Poolkhet, Pattra Moonjit, Rungrueang Yodsheewan, Thaweesak Songserm
• Journal: Asian Pacific journal of allergy and immunology (Asian Pac J Allergy Immunol) Vol. 28 Issue 4 Pg. 294-301 (Dec 2010) ISSN: 0125-877X [Print] Thailand
• PMID: 21337915 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antigens, Viral
• Sialic Acids

Topics

• Animals
• Antigens, Viral (immunology, metabolism)
• Birds
• Cats
• Disease Transmission, Infectious (prevention & control)
• Dogs
• Host-Pathogen Interactions
• Humans
• Immunohistochemistry
• Influenza A virus (immunology, pathogenicity)
• Influenza in Birds (immunology)
• Influenza, Human (immunology)
• Lung (immunology, metabolism, virology)
• Sialic Acids (immunology, metabolism)
• Species Specificity
• Trachea (immunology, metabolism, virology)
• Virulence