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Effects of propofol on proliferation and anti-apoptosis of neuroblastoma SH-SY5Y cell line: new insights into neuroprotection.

Abstract
Recently, it has been suggested that anesthetic agents may have neuroprotective potency. The notion that anesthetic agents can offer neuroprotection remains controversial. Propofol, which is a short-acting intravenous anesthetic agent, may have potential as a neuroprotective agent. In this study, we tried to determine whether propofol affected the viability of human neuroblastoma SH-SY5Y cells by using the MTT assay. Surprisingly, our results showed that propofol at a dose of 1-10 μM could improve cell proliferation. However, at higher doses (200 μM), propofol appears to be cytotoxic. On the other hand, propofol could up-regulate the expression of key proteins involved in neuroprotection including B-cell lymphoma 2 at a dose range of 1-10 μM, activation of phospho-serine/threonine protein kinase at a dose range of 0.5-10 μM, and activation of phospho-extracellular signal-regulated kinases at a dose range of 5-10 μM. Similarly, we demonstrate that propofol (10 μM) could elevate protein levels of heat shock protein 90 and heat shock protein 70. Therefore, we choose to utilize a 10 μM concentration of propofol to assess neuroprotective activities in our studies. In the following experiments, we used dynorphin A to generate cytotoxic effects on SH-SY5Y cells. Our data indicate that propofol (10 μM) could inhibit the cytotoxicity in SH-SY5Y cells induced by dynorphin A. Furthermore, propofol (10 μM) could decrease the expression of the p-P38 protein as well. These data together suggest that propofol may have the potential to act as a neuroprotective agent against various neurologic diseases. However, further delineation of the precise neuroprotective effects of propofol will need to be examined.
AuthorsGong-Jhe Wu, Wu-Fu Chen, Han-Chun Hung, Yen-Hsuan Jean, Chun-Sung Sung, Chiranjib Chakraborty, Hsin-Pai Lee, Nan-Fu Chen, Zhi-Hong Wen
JournalBrain research (Brain Res) Vol. 1384 Pg. 42-50 (Apr 12 2011) ISSN: 1872-6240 [Electronic] Netherlands
PMID21315692 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
CopyrightCopyright © 2011 Elsevier B.V. All rights reserved.
Chemical References
  • Heat-Shock Proteins
  • Neuroprotective Agents
  • Neurotransmitter Agents
  • Dynorphins
  • Propofol
Topics
  • Apoptosis (drug effects)
  • Cell Line, Tumor
  • Cell Proliferation (drug effects)
  • Dose-Response Relationship, Drug
  • Dynorphins (pharmacology)
  • Gene Expression Regulation, Neoplastic (drug effects)
  • Heat-Shock Proteins (genetics, metabolism)
  • Humans
  • In Situ Nick-End Labeling (methods)
  • Neuroblastoma (pathology)
  • Neuroprotective Agents (pharmacology)
  • Neurotransmitter Agents (pharmacology)
  • Propofol (pharmacology)
  • Time Factors

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