Abstract | OBJECTIVES: METHODS:
Chronic pancreatitis model was induced by trinitrobenzene sulfonic acid infusion into rat pancreatic ducts. Abdominal hyperalgesia was measured by thermal withdrawal latencies. The expression of PAR2 and transient receptor potential vanilloid 1 (TRPV1) were analyzed by immunofluorescence and Western blot. The messenger RNA encoding PAR2 was quantitated by real-time polymerase chain reaction. The effects of short-term and long-term ulinastatin treatment on abdominal thermal hyperalgesia of rats with CP were measured. RESULTS: Rats with CP showed a decreased thermal withdrawal latency. Proteinase-activated receptor 2 and TRPV1 were significantly upregulated in DRGs. The increased PAR2 protein expression was tightly correlated with thermal withdrawal latencies and TRPV1 expression. Short-term ulinastatin treatment inhibited the development of thermal hyperalgesia of rats with CP in a dose-dependent manner. CONCLUSIONS:
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Authors | Wei Zhang, Jun Gao, Tao Zhao, Lei Wei, Wenbin Wu, Yu Bai, Duowu Zou, Zhaoshen Li |
Journal | Pancreas
(Pancreas)
Vol. 40
Issue 2
Pg. 300-7
(Mar 2011)
ISSN: 1536-4828 [Electronic] United States |
PMID | 21311307
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Analgesics
- Glycoproteins
- RNA, Messenger
- Receptor, PAR-2
- TRPV Cation Channels
- Trpv1 protein, rat
- Trinitrobenzenesulfonic Acid
- urinastatin
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Topics |
- Abdominal Pain
(etiology, metabolism, physiopathology, prevention & control)
- Analgesics
(pharmacology)
- Animals
- Behavior, Animal
- Blotting, Western
- Disease Models, Animal
- Dose-Response Relationship, Drug
- Fluorescent Antibody Technique
- Ganglia, Spinal
(metabolism, physiopathology)
- Glycoproteins
(pharmacology)
- Hyperalgesia
(etiology, metabolism, physiopathology, prevention & control)
- Male
- Pain Measurement
- Pain Threshold
- Pancreatitis, Chronic
(chemically induced, complications, metabolism, physiopathology)
- RNA, Messenger
(metabolism)
- Rats
- Rats, Sprague-Dawley
- Reaction Time
- Receptor, PAR-2
(genetics, metabolism)
- Reverse Transcriptase Polymerase Chain Reaction
- TRPV Cation Channels
(metabolism)
- Time Factors
- Trinitrobenzenesulfonic Acid
- Up-Regulation
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