Platelet binding and activity of a factor VIIa variant with enhanced tissue factor independent activity.

Abstract

BACKGROUND AND OBJECTIVES:

Platelet binding and activity play important roles in the efficacy of factor VIIa (FVIIa) as a bypassing agent for hemophilia treatment. An analog of FVIIa with increased tissue factor (TF)-independent activity, NN1731, has been produced by introducing three amino acid changes in the protease domain. NN1731 has a conformation similar to TF-bound FVIIa, even in the absence of TF. This results in much greater intrinsic proteolytic activity, but similar activity in the presence of TF.

OBJECTIVES:

We hypothesized that these changes would not alter binding to platelets or phospholipid, a characteristic thought to be localized to the Gla domain. The goal of the current work was to compare platelet binding and activity of NN1731 and wild-type FVIIa.

METHODS/RESULTS:

FVIIa and NN1731 bound identically to phospholipid vesicles as assessed by both activity assays and electrophoretic quasielastic light scattering techniques. However, NN1731 bound to a greater number of sites on activated platelets than FVIIa, as assessed by flow cytometry. Removal of the Gla domain abolished binding of both FVIIa and NN1731. Inhibition of the active site did not reduce NN1731 binding to the level of FVIIa. When corrected for the amount of protein bound, NN1731 had greater activity than FVIIa on platelet surfaces.

CONCLUSIONS:

While the Gla domain is essential for FVIIa binding to platelets, changes in the protease domain in NN1731 enhanced platelet binding as well as proteolytic activity. Features in addition to lipid composition appear to contribute to binding of rFVIIa and, especially, NN1731 to platelets.

• Authors: M Hoffman, Z Volovyk, E Persson, D A Gabriel, M Ezban, D M Monroe
• Journal: Journal of thrombosis and haemostasis : JTH (J Thromb Haemost) Vol. 9 Issue 4 Pg. 759-66 (Apr 2011) ISSN: 1538-7836 [Electronic] England
• PMID: 21294824 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2011 International Society on Thrombosis and Haemostasis.

Chemical References

• Thromboplastin
• Factor VIIa

Topics

• Adult
• Blood Platelets (cytology)
• Factor VIIa (metabolism)
• Humans
• Middle Aged
• Thromboplastin (metabolism)