Abstract | AIMS: MAIN METHODS: We employed CHO cell lines expressing vector and SR-BI to test the effect of SR-BI on apoptosis induced by deficiency of calcium, magnesium and zinc in culture medium. The regain of different metal ions in deficient medium was also performed, respectively. Cell death was detected by morphological changes and quantified by LDH cytotoxicity assay. Apoptosis was also assessed by DNA ladder assay and DNA condensation assay. The SR-BIC323G mutant cells which lack the apoptotic activity of SR-BI were employed to verify the SR-BI-dependent effect on calcium/ magnesium induced apoptosis. KEY FINDINGS: The deficiency of calcium/ magnesium induced cell apoptosis in CHO-SR-BI cells, but not in CHO-vector cells. Moreover, no apoptotic cell death was observed in SR-BIC323G mutant cells, indicating that the deficiency of divalent metal ions induces apoptosis in a SR-BI-dependent manner. Furthermore, the restoration of calcium or magnesium, but not zinc, protected CHO-SR-BI cells from apoptotic cell death, in a dose-dependent fashion. SIGNIFICANCE:
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Authors | Hong Feng, Ling Guo, Haiqing Gao, Xiang-An Li |
Journal | Life sciences
(Life Sci)
Vol. 88
Issue 13-14
Pg. 606-12
(Mar 28 2011)
ISSN: 1879-0631 [Electronic] Netherlands |
PMID | 21291896
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2011 Elsevier Inc. All rights reserved. |
Chemical References |
- Culture Media
- SCARB1 protein, human
- Scavenger Receptors, Class B
- Magnesium
- Calcium
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Topics |
- Animals
- Apoptosis
(genetics, physiology)
- CHO Cells
- Calcium
(deficiency)
- Cell Culture Techniques
- Cell Nucleus
(metabolism, pathology)
- Cell Survival
(genetics)
- Cloning, Molecular
- Cricetinae
- Cricetulus
- Culture Media
- DNA Fragmentation
- Electrophoresis, Agar Gel
- Humans
- Magnesium
(metabolism)
- Magnesium Deficiency
- Mutation
- Scavenger Receptors, Class B
(genetics, physiology)
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