Proliferative responses of Brugia malayi TPX-1 and its epitopic peptide(29-43) in an endemic population of human lymphatic filariasis.

Abstract

Although the antioxidant thioredoxin peroxidase (TPX) is a putative target exploited in vaccine studies of lymphatic filariasis, the high sequence homology with host peroxiredoxins remains a great concern. The emergence of immunomics offers a powerful tool for novel vaccine design. Further, due to the cellular hypo-response in filariasis, analysis of T epitope repertoire becomes imperative in disease control. Here, we report the cellular responses of filarial TPX-1 and the identification of T epitope (29-43) in the host non-homologous region. The strong proliferative responses induced by the peptide mimetic in mice splenocytes and human PBMC's prove the existence of T epitope recognized in endemic population.

• Authors: Jayaprakasam Madhumathi, Gandhirajan Anugraha, Prabhu Rajaiah Prince, Dhinakar Pradiba, Perumal Kaliraj
• Journal: Microbes and infection (Microbes Infect) Vol. 13 Issue 6 Pg. 602-6 (Jun 2011) ISSN: 1769-714X [Electronic] France
• PMID: 21288496 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2011 Institut Pasteur. Published by Elsevier SAS. All rights reserved.

Chemical References

• Antigens, Helminth
• Epitopes, T-Lymphocyte
• Peroxiredoxins

Topics

• Animals
• Antigens, Helminth (immunology)
• Brugia malayi (immunology)
• Elephantiasis, Filarial (immunology, parasitology)
• Epitopes, T-Lymphocyte (immunology)
• Female
• Humans
• Mice
• Mice, Inbred BALB C
• Peroxiredoxins (immunology)