[Inhibitive effect of ursolic acid on the invasion and metastasis of ovarian carcinoma cells HO-8910PM].

Abstract

OBJECTIVE:

To study the effects of ursolic acid (UA) on the invasion and metastasis of ovarian carcinoma cell HO-8910PM and its underlying mechanism.

METHODS:

MTT assay was performed to examine the effects of UA on the proliferation of ovarian carcinoma cells HO-8910PM in vitro. The effects of UA on the invasion and migration of HO-8910PM were evaluated using Transwell chambers attached with polycarbonate filters and reconstituted basement membrane. Gelatin zymography was performed to detect the activity of gelatinase in the HO-8910PM cells. The expressions of MMP-2 and MMP-9 in the HO-8910PM cells were measured by Western blot.

RESULTS:

UA inhibited the proliferation of HO-8910PM cells in a time- and dose-dependent manner. There was a statistically significant difference in the invasion and migration of HO-8910PM cells between the UA treated cells and the controls (P < 0.01, P < 0.05). UA inhibited the activity of gelatinase of the treated cells.

CONCLUSION:

UA downregulated the expressions of MMP-2 and MMP-9. UA inhibits the invasion and metastasis of HO-8910PM cells, probably through inhibiting the activity of gelatinase and the expressions of MMP-2 and MMP-9.

• Authors: Li-bo Yu, Jing Wang, Bao-zhang Ma, Wen-zhou Sun
• Journal: Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition (Sichuan Da Xue Xue Bao Yi Xue Ban) Vol. 41 Issue 6 Pg. 986-8, 1038 (Nov 2010) ISSN: 1672-173X [Print] China
• PMID: 21265099 (Publication Type: English Abstract, Journal Article)

Chemical References

• Antineoplastic Agents
• Triterpenes
• MMP2 protein, human
• Matrix Metalloproteinase 2
• Matrix Metalloproteinase 9
• ursolic acid

Topics

• Antineoplastic Agents (pharmacology)
• Cell Line, Tumor
• Female
• Humans
• Matrix Metalloproteinase 2 (metabolism)
• Matrix Metalloproteinase 9 (metabolism)
• Neoplasm Invasiveness (prevention & control)
• Neoplasm Metastasis (prevention & control)
• Ovarian Neoplasms (pathology)
• Triterpenes (pharmacology)