Abstract | BACKGROUND AND AIM: METHODS: IL-10(-/-) and wild-type mice were used at 8 weeks of age and LP by gavage was administered at a dose of 10(9) cells/day per mice for 4 weeks. Mice were maintained for another one week without LP treatment. The colonic tissues were collected for histological and ultrastructural analysis at death after 4 weeks treatment of LP, and the feces were collected at 1-week intervals throughout the experiment for the analysis of gut flora and LP using selective culture-based techniques. RESULTS: Compared with control mice, IL-10(-/-) mice developed a severe intestinal inflammation and tissue damage, and had an abnormal composition of gut microflora. LP administration attenuated colitis with the decreased inflammatory scoring and histological injury in the colon of IL-10(-/-) mice. In addition, LP administration increased the numbers of beneficial total bifidobacteria and lactobacilli, and decreased the numbers of potential pathogenic enterococci and Clostridium perfringens, although the decrease of coliforms was not significant after LP treatment in IL-10(-/-) mice. CONCLUSIONS:
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Authors | Yang Xia, Hong-Qi Chen, Min Zhang, Yan-Qun Jiang, Xiao-Min Hang, Huan-Long Qin |
Journal | Journal of gastroenterology and hepatology
(J Gastroenterol Hepatol)
Vol. 26
Issue 2
Pg. 405-11
(Feb 2011)
ISSN: 1440-1746 [Electronic] Australia |
PMID | 21261733
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | © 2011 Journal of Gastroenterology and Hepatology Foundation and Blackwell Publishing Asia Pty Ltd. |
Chemical References |
- IL10 protein, mouse
- Interleukin-10
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Topics |
- Administration, Oral
- Animals
- Bifidobacterium
(growth & development)
- Clostridium perfringens
(growth & development)
- Colitis
(genetics, immunology, microbiology, pathology, prevention & control)
- Colon
(immunology, microbiology, ultrastructure)
- Disease Models, Animal
- Enterococcus
(growth & development)
- Feces
(microbiology)
- Female
- Interleukin-10
(deficiency, genetics)
- Lactobacillus plantarum
(growth & development)
- Mice
- Mice, 129 Strain
- Mice, Knockout
- Probiotics
(administration & dosage)
- Severity of Illness Index
- Time Factors
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