(1→3)-β-D-Glucan inhibits a dual mechanism of peroxynitrite stroke.

Abstract

AIM:

The antioxidative and antinitrative activities of (1→3)-β-D-glucan (1-4μg/ml) from the yeast cell walls of Saccharomyces cerevisiae in human plasma treated with strong oxidants - peroxynitrite (ONOO(-)) (0.1mM) and hydrogen peroxide (H(2)O(2)) (2mM) were studied in vitro. The main purpose of this study was to assess if (1→3)-β-D-glucan, a well known strong immunostimulatory agent, possesses a protective function against dual mechanism of ONOO(-) stroke associated with nitrative and oxidative damages to human plasma biomolecules.

SCOPE:

The protein changes were determined in vitro by estimating the level of oxidative stress markers - carbonyl groups, and nitrative products - 3-nitrotyrosine residues. The plasma lipid peroxidation was also investigated. The obtained results show that (1→3)-β-D-glucan inhibits in vitro ONOO(-)-induced oxidation and nitration of plasma proteins, even by 50% and 30%, respectively. The antioxidative activity of (1→3)-β-D-glucan was confirmed by its inhibitory effect on plasma lipids peroxidation induced by ONOO(-) or by H(2)O(2).

CONCLUSIONS:

The obtained results demonstrate that (1→3)-β-D-glucan from S. cerevisiae protects plasma components against toxic effects of ONOO(-) and H(2)O(2) due to its antioxidative and antinitrative activities. Therefore (1→3)-β-D-glucan supplementation during inflammatory may be beneficial not only regard for its ability to stimulate the immune system but also by antioxidative properties.

• Authors: Joanna Saluk-Juszczak, Karolina Krolewska, Barbara Wachowicz
• Journal: International journal of biological macromolecules (Int J Biol Macromol) Vol. 48 Issue 3 Pg. 488-94 (Apr 01 2011) ISSN: 1879-0003 [Electronic] Netherlands
• PMID: 21255603 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2011 Elsevier B.V. All rights reserved.

Chemical References

• Antioxidants
• Blood Proteins
• Oxidants
• beta-Glucans
• Peroxynitrous Acid
• Hydrogen Peroxide

Topics

• Adult
• Antioxidants (chemistry, metabolism, pharmacology)
• Blood Proteins (drug effects)
• Humans
• Hydrogen Peroxide (metabolism, pharmacology)
• Lipid Peroxidation (drug effects)
• Male
• Oxidants (metabolism, pharmacology)
• Oxidation-Reduction (drug effects)
• Oxidative Stress (drug effects)
• Peroxynitrous Acid (metabolism, pharmacology)
• Plasma (chemistry, drug effects)
• Saccharomyces cerevisiae (chemistry)
• Young Adult
• beta-Glucans (chemistry, metabolism, pharmacology)