Abstract | BACKGROUND: METHODS: Tissue microarrays were immunohistochemically stained for claudin-3, with the staining intensities subsequently quantified and statistically analyzed using a one-way ANOVA with subsequent Tukey tests for multiple comparisons or a nonparametric equivalent. Fifty-three cases of NAC, 17 cases of BPH, 35 cases of PIN, 107 cases of PCa, and 55 cases of Mets were analyzed in the microarrays. RESULTS: PCa and Mets had the highest absolute staining for claudin-3. Both had significantly higher staining than BPH (p < 0.05 in both cases) and NAC (p < 0.05 in both cases). PIN had a lower, but non-significant, staining score than PCa and Mets, but a statistically higher score than both BPH and NAC (p < 0.05 for both cases). No significant differences were observed between PCa, Mets, and PIN. CONCLUSIONS: To our knowledge, this represents one of the first studies comparing the immunohistochemical profiles of claudin-3 in PCa and NAC to specimens of PIN, BPH, and Mets. These findings provide further evidence that claudin-3 may serve as an important biomarker for prostate cancer, both primary and metastatic, but does not provide evidence that claudin-3 can be used to predict risk of metastasis.
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Authors | Tanner L Bartholow, Uma R Chandran, Michael J Becich, Anil V Parwani |
Journal | Diagnostic pathology
(Diagn Pathol)
Vol. 6
Pg. 12
(Jan 21 2011)
ISSN: 1746-1596 [Electronic] England |
PMID | 21255442
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
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Chemical References |
- Biomarkers, Tumor
- CLDN3 protein, human
- Claudin-3
- Membrane Proteins
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Topics |
- Adenocarcinoma
(metabolism, pathology, secondary)
- Biomarkers, Tumor
(metabolism)
- Biopsy
- Claudin-3
- Humans
- Male
- Membrane Proteins
(metabolism)
- Prostate
(metabolism, pathology)
- Prostatic Hyperplasia
(metabolism, pathology)
- Prostatic Intraepithelial Neoplasia
(metabolism, pathology)
- Prostatic Neoplasms
(metabolism, pathology, secondary)
- Protein Array Analysis
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