Application of porous membrane protected micro-solid-phase-extraction combined with gas chromatography-mass spectrometry for the determination of estrogens in ovarian cyst fluid samples.
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| Abstract |
A cost effective and environmentally friendly extraction technique using porous membrane protected micro-solid phase extraction (μ-SPE) is described for the extraction of estrogens in cyst fluid samples obtained from cancer patients. A sorbent (ethylsilane (C2) modified silica) (20 mg) was packed in a porous polypropylene envelope (2 cm×1.5 cm) whose edges were heat sealed to secure the contents. The μ-SPE device was conditioned with acetone and placed in a stirred (1:5) diluted cyst fluid sample solution (10 mL) to extract estrogens for 60 min. After extraction, the analytes were desorbed and simultaneously derivatized with a 5:1 mixture of acetone and N,O-bis(trimethylsilyl)-trifluoroacetamide. The extract (2 μL) was analyzed by gas chromatography-mass spectrometry. Various extraction, desorption and derivatization conditions were optimized for μ-SPE. With this simple technique, low limits of detection of between 9 and 22 ng L(-1) and linear range from the detection limits up to 50 μg L(-1) were achieved. The optimized method was used to extract estrogens from cyst fluid samples obtained from patients with malignant and benign ovarian tumors.
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| Authors | Sivarajan Kanimozhi, Chanbasha Basheer, Kothandaraman Narasimhan, Lin Liu, Stephen Koh, Feng Xue, Mahesh Choolani, Hian Kee Lee |
| Journal | Analytica chimica acta (Anal Chim Acta) Vol. 687 Issue 1 Pg. 56-60 (Feb 14 2011) ISSN: 1873-4324 [Electronic] Netherlands |
| PMID | 21241846 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't) |
| Copyright | Copyright © 2010 Elsevier B.V. All rights reserved. |
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