Development of a capture ELISA to determine kinetics of soluble CD25 following in vitro and in vivo stimulation of duck peripheral blood monocytes.

Abstract

In humans and other mammals, the α-chain of interleukin-2 (IL-2) receptor (CD25) is induced and expressed on the cell surface after lymphocyte activation and is released from the membrane of activated cells as a smaller soluble form (sCD25). However, little is known about avian sCD25. In the present study, we developed an antigen capture enzyme-linked immunosorbent assay (AC-ELISA) to detect serum sCD25 in ducks, and we used flow cytometry (FCM) to analyze the frequency of CD25(+) cells in the peripheral blood of ducks infected with H9N2 or H5N1 avian influenza virus (AIV) or serotype II Riemerella anatipestifer (RA). Using the AC-ELISA, duck sCD25 molecules were detected in the supernatant and lysates of concanavalin A (Con A)-stimulated peripheral blood mononuclear cells (PBMC), and in the serum of ducks infected with H5N1 virus and RA. However, no sCD25 was detected in the serum of H9N2 AIV-infected ducks. FCM analysis revealed that CD25(+) cells were upregulated within the PBMC of RA-infected ducks throughout the experiment until death, while in the PBMC of H9N2- and H5N1 AIV-infected ducks, the frequency of CD25(+) cells increased in the early stage of infection and then returned to a lower level. Our findings confirm that the dynamics of sCD25 and CD25(+) cells are different in the peripheral blood of ducks infected with H9N2 virus, H5N1 virus, and RA.

• Authors: Zhenyu Huang, Jie Fang, Jianyou Gu, Yan Yan, Jiyong Zhou
• Journal: Veterinary immunology and immunopathology (Vet Immunol Immunopathol) Vol. 140 Issue 1-2 Pg. 102-9 (Mar 15 2011) ISSN: 1873-2534 [Electronic] Netherlands
• PMID: 21216015 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2010 Elsevier B.V. All rights reserved.

Chemical References

• Interleukin-2 Receptor alpha Subunit
• Concanavalin A

Topics

• Animals
• Concanavalin A (immunology)
• Ducks (blood, immunology, microbiology, virology)
• Enzyme-Linked Immunosorbent Assay (methods, veterinary)
• Flavobacteriaceae (immunology)
• Flavobacteriaceae Infections (immunology, veterinary)
• Flow Cytometry (veterinary)
• Influenza A Virus, H5N1 Subtype (immunology)
• Influenza A Virus, H9N2 Subtype (immunology)
• Influenza in Birds (immunology)
• Interleukin-2 Receptor alpha Subunit (biosynthesis, blood)
• Kinetics
• Leukocytes, Mononuclear (metabolism)
• Lymphocyte Count (veterinary)
• T-Lymphocytes (metabolism)