Abstract | BACKGROUND: OBJECTIVES: The goal was to conduct proteomic profiling of patients with AD based on S. aureus colonization status and history of eczema herpeticum. We hoped to identify new biomarkers for improved diagnosis and prediction of eczema herpeticum and S. aureus susceptibility and to generate new hypotheses regarding disease pathogenesis. METHODS: Skin taping was performed on nonlesional skin of nonatopic control subjects and on lesional and nonlesional skin of patients with AD. Subjects were classified according to the history of eczema herpeticum and S. aureus colonization. Proteins were analyzed by using mass spectrometry; diagnostic groups were compared for statistically significant differences in protein expression. RESULTS: CONCLUSION: This noninvasive, semiquantitative profiling method has revealed novel proteins likely involved in the pathogenesis of AD. The lower expression of skin barrier proteins and enzymes involved in the generation of the natural moisturizing factor could further exacerbate barrier defects and perpetuate water loss from the skin. The greater expression of epidermal fatty acid-binding protein, especially in patients colonized with methicillin-resistant S. aureus, might perpetuate the inflammatory response through eicosanoid signaling.
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Authors | Carolyn J Broccardo, Spencer Mahaffey, John Schwarz, Lisa Wruck, Gloria David, Patrick M Schlievert, Nichole A Reisdorph, Donald Y M Leung |
Journal | The Journal of allergy and clinical immunology
(J Allergy Clin Immunol)
Vol. 127
Issue 1
Pg. 186-93, 193.e1-11
(Jan 2011)
ISSN: 1097-6825 [Electronic] United States |
PMID | 21211653
(Publication Type: Comparative Study, Journal Article, Research Support, N.I.H., Extramural)
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Copyright | Copyright © 2010 American Academy of Allergy, Asthma & Immunology. Published by Mosby, Inc. All rights reserved. |
Topics |
- Adult
- Dermatitis, Atopic
(complications, metabolism, microbiology)
- Female
- Filaggrin Proteins
- Humans
- Kaposi Varicelliform Eruption
(complications, metabolism)
- Male
- Mass Spectrometry
- Proteomics
- Skin
(metabolism)
- Staphylococcal Infections
(complications, metabolism)
- Staphylococcus aureus
- Young Adult
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