Photodynamic therapy with the silicon phthalocyanine pc 4 induces apoptosis in mycosis fungoides and sezary syndrome.

Abstract

Our current focus on the effects of Photodynamic Therapy (PDT) using silicon phthalocyanine Pc 4 photosensitizer on malignant T lymphocytes arose due to preclinical observations that Jurkat cells, common surrogate for human T cell lymphoma, were more sensitive to Pc 4-PDT-induced killing than epidermoid carcinoma A431 cells. Mycosis fungoides (MF) as well as Sezary syndrome (SS) are variants of cutaneous T-cell lymphoma (CTCL) in which malignant T-cells invade the epidermis. In this study, we investigated the cytotoxicity of Pc 4-PDT in peripheral blood cells obtained from patients with SS and in skin biopsies of patients with MF. Our data suggest that Pc 4-PDT preferentially induces apoptosis of CD4(+)CD7(-) malignant T-lymphocytes in the blood relative to CD11b(+) monocytes and nonmalignant T-cells. In vivo Pc 4-PDT of MF skin also photodamages the antiapoptotic protein Bcl-2.

• Authors: Minh Lam, Yoojin Lee, Min Deng, Andrew H Hsia, Kelly A Morrissey, Chunlin Yan, Kashif Azzizudin, Nancy L Oleinick, Thomas S McCormick, Kevin D Cooper, Elma D Baron
• Journal: Advances in hematology (Adv Hematol) Vol. 2010 Pg. 896161 ( 2010) ISSN: 1687-9112 [Electronic] United States
• PMID: 21197103 (Publication Type: Journal Article)