Abstract | OBJECTIVE: This study investigates the role of mannose-binding lectin (MBL) in the susceptibility to HIV-1 infection analyzing polymorphisms located at the MBL2 promoter and exon 1 regions. MATERIALS AND METHODS: The prevalence of MBL2 variant alleles was investigated in 410 HIV-1-infected patients from the South Brazilian HIV cohort and in 345 unexposed uninfected healthy individuals. The promoter variants were genotyped using polymerase chain reaction with sequence-specific primers (PCR-SSP) and exon 1 variants were analyzed by real-time PCR using a melting temperature assay and were confirmed by PCR-restriction fragment length polymorphism (RFLP). MBL2 genotypic and allelic frequencies were compared between HIV-1-infected patients and controls using the chi-squared tests. RESULTS: The analyses were performed subdividing the individuals according to their ethnic origin. Among Euro-derived individuals a higher frequency of the LX/LX genotype was observed in patients when compared to controls (P < 0.001). The haplotypic analysis also showed a higher frequency of the haplotypes associated with lower MBL levels among HIV-1-infected patients (P = 0.0001). Among Afro-derived individuals the frequencies of LY/LY and HY/HY genotypes were higher in patients when compared to controls (P = 0.009 and P = 0.02). CONCLUSIONS: An increased frequency of MBL2 genotypes associated with low MBL levels was observed in Euro-derived patients, suggesting a potential role for MBL in the susceptibility to HIV-1 infection in Euro-derived individuals.
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Authors | Gabriela Kniphoff da Silva, Rafael Guimarães, Vanessa Suñé Mattevi, Rosmeri Kuhmmer Lazzaretti, Eduardo Sprinz, Regina Kuhmmer, Lucas Brandão, Sérgio Crovella, José Artur Bogo Chies |
Journal | AIDS (London, England)
(AIDS)
Vol. 25
Issue 4
Pg. 411-8
(Feb 20 2011)
ISSN: 1473-5571 [Electronic] England |
PMID | 21192229
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Lectins, C-Type
- MBL2 protein, human
- Mannose Receptor
- Mannose-Binding Lectin
- Mannose-Binding Lectins
- Receptors, Cell Surface
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Topics |
- Adult
- Aged
- Brazil
(epidemiology)
- Female
- Genetic Predisposition to Disease
(genetics)
- HIV Infections
(epidemiology, genetics, immunology)
- HIV-1
- Humans
- Lectins, C-Type
(genetics, immunology)
- Male
- Mannose Receptor
- Mannose-Binding Lectin
(genetics, immunology)
- Mannose-Binding Lectins
(genetics, immunology)
- Middle Aged
- Polymorphism, Genetic
- Promoter Regions, Genetic
(genetics)
- Receptors, Cell Surface
(genetics, immunology)
- Young Adult
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