[Proteomic analysis of hippocampus in the rat].

Abstract

AIM:

To initially analyze the protein expression in the rat hippocampus with the proteomics approach.

METHODS:

Proteins from hippocampal tissue homogenates of the rat were separated by two-dimensional gel electrophoresis(2-DE), and the proteins were stained with colloidal coomassie blue to produce a high-resolution map of the rat hippocampus proteome. Selected proteins from this map were digested with trypsin, and the resulting tryptic peptides were analyzed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS). The mass spectrometric data were used to identify the proteins through searches of the NCBI protein sequence database.

RESULTS:

37 prominent proteins with various functional characteristics were identified. The identification of brain protein classes, like metabolism enzymes, cytoskeleton proteins, heat shock proteins, antioxidant proteins, signalling proteins, proteasome-related proteins, neuron-specific proteins and glial-associated proteins. Furthermore, 3 hypothetical proteins which are unknown proteins, so far only proposed from their nucleic acid structure were identified.

CONCLUSION:

This study provides the first unbiased characterization of proteins of the rat hippocampus and will be used for future studies of differential protein expression in rat models of neurological disorders.

• Authors: Bo Zhang, Ren-Zhi Wang, Ming Fan, Yong Yao, Gui-Lin Li
• Journal: Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology (Zhongguo Ying Yong Sheng Li Xue Za Zhi) Vol. 22 Issue 1 Pg. 26-9 (Feb 2006) ISSN: 1000-6834 [Print] China
• PMID: 21186571 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Proteome

Topics

• Animals
• Electrophoresis, Gel, Two-Dimensional
• Hippocampus (metabolism)
• Male
• Neurons (metabolism)
• Proteome (analysis)
• Proteomics
• Rats
• Rats, Sprague-Dawley