Abstract |
Activation of anti-apoptotic gene transcription by NF-κB ( nuclear factor-kappa B) has been reported to be linked with a resistance of cancer cells against chemotherapy. NEMO (NF-κB essential modulator) interacts with a number of proteins and modulates the activity of NF-κB pathway. In this study, we revealed that RPAP3 ( RNA polymerase II-associated protein 3) possesses an activity to bind with NEMO and to inhibit the ubiquitination of NEMO and that RPAP3 enhances doxorubicin-induced cell death in breast cancer cell line T-47D through the marked impairment of NF-κB pathway. These results indicate that RPAP3 may be a novel modulator of NF-κB pathway in apoptosis induced by anti- cancer chemotherapeutic agents.
|
Authors | Kana Shimada, Makio Saeki, Hiroshi Egusa, Sho Fukuyasu, Yoshiaki Yura, Kazuhiro Iwai, Yoshinori Kamisaki |
Journal | Biochemical and biophysical research communications
(Biochem Biophys Res Commun)
Vol. 404
Issue 4
Pg. 910-4
(Jan 28 2011)
ISSN: 1090-2104 [Electronic] United States |
PMID | 21184742
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
|
Copyright | Copyright © 2010 Elsevier Inc. All rights reserved. |
Chemical References |
- Antibiotics, Antineoplastic
- Apoptosis Regulatory Proteins
- Carrier Proteins
- IKBKG protein, human
- NF-kappa B
- RPAP3 protein, human
- Doxorubicin
- I-kappa B Kinase
|
Topics |
- Antibiotics, Antineoplastic
(pharmacology)
- Apoptosis
- Apoptosis Regulatory Proteins
- Carrier Proteins
(genetics, metabolism)
- Cell Line, Tumor
- Doxorubicin
(pharmacology)
- Humans
- I-kappa B Kinase
(metabolism)
- NF-kappa B
(metabolism)
- Phosphorylation
- Ubiquitination
|