Abstract | BACKGROUND: METHODS: Human endothelial cells EA.hy926 were treated with tumour necrosis factor-α (TNFα) in the presence or not of Avène TSW during 4 h. As nuclear factor-κB (NF-κB) is involved in the signalisation of inflammatory mediators such as the adhesion molecules, the translocation of NF-κB in endothelial cells was assessed by immunohistochemistry with anti-NF-κBp65. The protein and mRNA levels of TNFα-induced ICAM-1 and E-selectin were assessed by ELISA assay and RT-PCR. These adhesion molecules were also detected by immunohistochemistry. RESULTS: Tumour necrosis factor-α induced the activation of p65 NF-κB nuclear translocation. TNFα also induced E-selectin and ICAM-1 in a dose-dependant manner in EA.hy926 endothelial cells. In the presence of Avène TSW, a significant inhibition of the TNFα-induced E-selectin and ICAM-1 expression (-22% and -7%, respectively, P < 0.05) was observed. CONCLUSION: These data suggest that Avène TSW mediated inhibition of TNFα-induced E-selectin and ICAM-1 expression. The inhibition of such adhesion molecules is attributable to the suppression of NF-κB transcription factor pathway activation.
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Authors | N Castex-Rizzi, M Charveron, C Merial-Kieny |
Journal | Journal of the European Academy of Dermatology and Venereology : JEADV
(J Eur Acad Dermatol Venereol)
Vol. 25 Suppl 1
Pg. 6-11
(Feb 2011)
ISSN: 1468-3083 [Electronic] England |
PMID | 21175868
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | © 2010 The Authors. JEADV © 2010 European Academy of Dermatology and Venereology. |
Chemical References |
- E-Selectin
- Mineral Waters
- NF-kappa B
- Tumor Necrosis Factor-alpha
- Intercellular Adhesion Molecule-1
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Topics |
- Analysis of Variance
- Cells, Cultured
- E-Selectin
(metabolism)
- Endothelial Cells
(metabolism)
- Enzyme-Linked Immunosorbent Assay
- Humans
- Intercellular Adhesion Molecule-1
(metabolism)
- Mineral Waters
(administration & dosage)
- NF-kappa B
(metabolism, physiology)
- Signal Transduction
(drug effects)
- Statistics, Nonparametric
- Tumor Necrosis Factor-alpha
(administration & dosage)
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