Aberrant expression and localization of hnRNP-A2/B1 is a common event in human gastric adenocarcinoma.

Abstract

BACKGROUND AND AIM:

Nuclear-matrix proteins can be proteomic markers for cancer lesions. The present study aimed to determine the roles of heterogeneous nuclear ribonucleoproteins--A2 and B1 (hnRNP-A2/B1) in human gastric carcinogenesis.

METHODS:

Human gastric cancer and non-cancerous tissues were collected for immunohistochemical analysis. Proteomics technique, Western blot, laser confocal microscope, and real-time quantitative reverse transcription-polymerase chain reaction were performed to determine the aberrant expression of nuclear-matrix proteins.

RESULTS:

hnRNP-A2/B1 existed in the nuclear matrix of gastric cancer cells, and its expression was enhanced in human gastric cancer and decreased by hexamethylene bisacetamide. The colocalization of hnRNP-A2/B1 with c-myc, c-fos, p53, and Rb was translocated from the nucleolus to the cytoplasm during the differentiation of tumor cells.

CONCLUSIONS:

hnRNP-A2/B1 affected tumor cell differentiation through interaction with oncogenes and tumor-suppressor genes, and it was overexpressed in human gastric cancer. We postulate that hnRNP-A2/B1 could serve as a biomarker for the diagnosis of human gastric cancer.

• Authors: Guang-Jun Jing, Dong-Hui Xu, Song-Lin Shi, Qi-Fu Li, San-Ying Wang, Fu-Yun Wu, Hai-Yan Kong
• Journal: Journal of gastroenterology and hepatology (J Gastroenterol Hepatol) Vol. 26 Issue 1 Pg. 108-15 (Jan 2011) ISSN: 1440-1746 [Electronic] Australia
• PMID: 21175803 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2010 Journal of Gastroenterology and Hepatology Foundation and Blackwell Publishing Asia Pty Ltd.

Chemical References

• Acetamides
• Biomarkers, Tumor
• Heterogeneous-Nuclear Ribonucleoprotein Group A-B
• MYC protein, human
• Proto-Oncogene Proteins c-fos
• Proto-Oncogene Proteins c-myc
• RNA, Messenger
• Retinoblastoma Protein
• TP53 protein, human
• Tumor Suppressor Protein p53
• hnRNP A2
• hexamethylene bisacetamide

Topics

• Acetamides (pharmacology)
• Adenocarcinoma (genetics, metabolism, pathology)
• Biomarkers, Tumor (genetics, metabolism)
• Blotting, Western
• Cell Differentiation (drug effects)
• Cell Line, Tumor
• China
• Cytoplasm (metabolism)
• Heterogeneous-Nuclear Ribonucleoprotein Group A-B (genetics, metabolism)
• Humans
• Immunohistochemistry
• Microscopy, Confocal
• Nuclear Matrix (metabolism)
• Prognosis
• Proteomics (methods)
• Proto-Oncogene Proteins c-fos (metabolism)
• Proto-Oncogene Proteins c-myc (metabolism)
• RNA, Messenger (metabolism)
• Retinoblastoma Protein (metabolism)
• Reverse Transcriptase Polymerase Chain Reaction
• Stomach Neoplasms (genetics, metabolism, pathology)
• Tumor Suppressor Protein p53 (metabolism)