Suppression of neuro inflammation in experimental autoimmune encephalomyelitis by glia maturation factor antibody.

Abstract

Glia maturation factor (GMF), a protein primarily localized in the central nervous system (CNS) was isolated, sequenced and cloned in our laboratory. We previously demonstrated that GMF mediates the experimental autoimmune encephalomyelitis (EAE)-induced production of pro-inflammatory cytokines and chemokines in the central nervous system of mice. In the present study we show that immunization with myelin oligodendrocyte glycoprotein peptide 35-55 (MOG35-55) caused an early onset (days 7-9 post immunization) and severe EAE with a mean peak score of 3.5 ± 0.5 in mice. Neutralization of GMF with four injections of anti-GMF antibody 5 to 11 days post immunization delayed the time of onset (days 12-14 post immunization) and significantly reduced the severity of EAE (mean peak score of 1.5 ± 0.4). Consistent with these clinical scores, histological examination of the CNS of these mice revealed profound differences between GMF-antibody treated mice and isotype matched control-antibody treated mice. Histological analysis of the spinal cord and brain showed severe inflammation and demyelination in the control antibody-treated mice whereas significantly reduced inflammation and demyelination was detected in GMF-antibody-treated mice at days 8, 16, and 24 post immunization. The decreased incidence and reduced severity of EAE in GMF-antibody-treated mice was consistent with the significantly reduced expressions of pro-inflammatory cytokines and chemokines. Our overall results demonstrate that neutralization of endogenous GMF with an affinity purified GMF antibody significantly decreased the inflammation, severity and progression of immunization induced active, passive and relapsing-remitting EAE. Treatment of mice with isotype-matched control antibody did not have any effect on EAE. Taken together, these results demonstrate the critical role of GMF in EAE, and GMF antibody as a potent anti-inflammatory therapeutic agent for effectively suppressing EAE in mouse models of major types of multiple sclerosis (MS).

• Authors: Smita Zaheer, Yanghong Wu, Shailendra K Sahu, Asgar Zaheer
• Journal: Brain research (Brain Res) Vol. 1373 Pg. 230-9 (Feb 10 2011) ISSN: 1872-6240 [Electronic] Netherlands
• PMID: 21146509 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, U.S. Gov't, Non-P.H.S.)
• Copyright: Published by Elsevier B.V.

Chemical References

• Antibodies
• Cytokines
• Glia Maturation Factor
• Glycoproteins
• Myelin-Oligodendrocyte Glycoprotein
• Peptide Fragments
• RNA, Messenger
• myelin oligodendrocyte glycoprotein (35-55)
• Pertussis Toxin

Topics

• Animals
• Antibodies (therapeutic use)
• Cytokines (genetics, metabolism)
• Encephalomyelitis, Autoimmune, Experimental (complications, etiology, pathology)
• Enzyme-Linked Immunosorbent Assay
• Gene Expression Regulation (drug effects, genetics)
• Glia Maturation Factor (immunology)
• Glycoproteins (adverse effects)
• Inflammation (drug therapy, etiology)
• Mice
• Mice, Inbred C57BL
• Myelin-Oligodendrocyte Glycoprotein
• Neurons (drug effects)
• Peptide Fragments (adverse effects)
• Pertussis Toxin (adverse effects)
• RNA, Messenger (metabolism)
• Time Factors